Jiangsu Key Laboratory of Pesticide Science, College of Sciences , Nanjing Agricultural University , Nanjing 210095 , China.
Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture , Nanjing Agricultural University , Nanjing 210095 , China.
J Agric Food Chem. 2018 Dec 19;66(50):13073-13083. doi: 10.1021/acs.jafc.8b04281. Epub 2018 Dec 4.
This study identified the effect of salicylic acid on degradation of isoproturon in Arabidopsis. Three T-DNA insertion mutant lines pal1- 1, pal1- 2, and eps1- 1 defective in salicylic acid synthesis were tested, which showed higher isoproturon accumulation and a toxic symptom in the mutants. When treated with 5 mg/L salicylic acid, these lines displayed a lower level of isoproturon and showed an attenuated toxic symptom. An RNA-sequencing study identified 2651 (1421 up and 1230 down) differentially expressed genes (DEGs) in eps1- 1 and 2211 (1556 up and 655 down) in pal1- 2 mutant plants (>2.0 fold change, p < 0.05). Some of the DEGs covered Phase I-III reaction components, like glycosyltransferases (GTs) and ATP-binding cassette transporters (ABCs). Using ultra performance liquid chromatography-time-of-flight-tandem-mass spectrometer/mass spectrometer (UPLC/Q-TOF-MS/MS), 13 Phase I and four Phase II metabolites were characterized. Of these, two metabolites 1-OH-isopropyl-benzene-O-glucoside and 4-isopropylphenol-S-2-methylbutanoyl-serine, have been identified and reported for the first time.
本研究鉴定了水杨酸对拟南芥中异丙隆降解的影响。测试了三个 T-DNA 插入突变体系 pal1-1、pal1-2 和 eps1-1，它们在水杨酸合成中存在缺陷，表现出更高的异丙隆积累和突变体中的毒性症状。当用 5mg/L 水杨酸处理时，这些系显示出较低水平的异丙隆，并表现出减轻的毒性症状。RNA 测序研究鉴定了 eps1-1 中的 2651 个（1421 个上调和 1230 个下调）差异表达基因（DEGs）和 pal1-2 突变体植物中的 2211 个（1556 个上调和 655 个下调）（>2.0 倍变化，p<0.05）。一些 DEGs 涵盖了 I 期-III 期反应成分，如糖基转移酶（GTs）和 ABC 转运蛋白。使用超高效液相色谱-飞行时间串联质谱/质谱（UPLC/Q-TOF-MS/MS），鉴定并表征了 13 种 I 期和 4 种 II 期代谢物。其中，两种代谢物 1-OH-异丙基苯-O-葡萄糖苷和 4-异丙基苯酚-S-2-甲基丁酰丝氨酸，被首次鉴定和报道。
