Laboratory of Structural Biology, Federal University of Para Institute of Biological Sciences, Belém, Pará, Brazil; Postgraduate Program in Biology of Infectious and Parasitic Agents, Federal University of Para Institute of Biological Sciences, Belém, Pará, Brazil; National Institute of Science and Technology in Structural Biology and Bioimaging, Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratory of Structural Biology, Federal University of Para Institute of Biological Sciences, Belém, Pará, Brazil.
Biomed Pharmacother. 2024 Oct;179:117360. doi: 10.1016/j.biopha.2024.117360. Epub 2024 Sep 3.
Macrophages undergo activation in response to multiple stimuli, including pathogens, growth factors and natural products. The inflammatory response and oxidative stress play critical roles in such macrophage activation. Some natural products reportedly promote immunoregulatory effects and the control of macrophage activation. Caryocar villosum (Cv), a native amazon plant, contains compounds that are an important source of molecules capable of macrophage activation. Herein, we demonstrate the immunomodulatory effects of oil obtained from Caryocar villosum (CvO) on macrophages. Macrophages were treated with varying concentrations of CvO, and resulting cellular morphological and functional changes were evaluated, including the production of nitric oxide (NO), reactive oxygen species (ROS), cytokines and phagocytic activity. Treatment of cells with 50 and 100 μg/mL CvO induced morphological and physiological alterations in the macrophages, such as increased cell surface and phagocytic activity. Additionally, treatment increased the productions of inflammatory cytokines (INF-γ, TNF-α, IL-6) and anti-inflammatory cytokines (IL-17 and IL-10) by macrophages, and significantly decreased ROS levels. In conclusion, these data suggest that, due to molecular diversity, CvO promoted an immunomodulatory effect on macrophages, mediated by an increased production of cytokines, and inhibition of ROS generation and phagocytic activity. Thus, CvO presents potential as a therapeutic agent for the treatment of inflammatory and non-inflammatory diseases.
巨噬细胞在多种刺激物（包括病原体、生长因子和天然产物）的作用下发生激活。炎症反应和氧化应激在这种巨噬细胞激活中发挥关键作用。一些天然产物据称可促进免疫调节作用和控制巨噬细胞的激活。Caryocar villosum（Cv）是一种原产于亚马逊的植物，其含有的化合物是能够激活巨噬细胞的分子的重要来源。在此，我们证明了从 Caryocar villosum（CvO）获得的油的免疫调节作用对巨噬细胞的影响。用不同浓度的 CvO 处理巨噬细胞，并评估细胞形态和功能的变化，包括一氧化氮（NO）、活性氧（ROS）、细胞因子和吞噬活性的产生。用 50 和 100 μg/mL 的 CvO 处理细胞会引起巨噬细胞的形态和生理变化，例如增加细胞表面和吞噬活性。此外，处理还会增加巨噬细胞产生炎症细胞因子（INF-γ、TNF-α、IL-6）和抗炎细胞因子（IL-17 和 IL-10），并显著降低 ROS 水平。总之，这些数据表明，由于分子多样性，CvO 通过增加细胞因子的产生、抑制 ROS 的产生和吞噬活性，对巨噬细胞产生了免疫调节作用。因此，CvO 具有作为治疗炎症和非炎症性疾病的治疗剂的潜力。
