甲状腺髓样癌的淀粉样纤维蛋白——生化及免疫化学特性

Amyloid fibril protein from medullary carcinoma of the thyroid--biochemical and immunochemical characterizations.

作者信息

Isobe T, Fukasa M, Fujita T

出版信息

Jpn J Med. 1985 Feb;24(1):8-12. doi: 10.2169/internalmedicine1962.24.8.

DOI:10.2169/internalmedicine1962.24.8

Abstract

A unique amyloid fibril protein (Nis) was extracted from a patient with medullary carcinoma of the thyroid (MCT). This amyloid protein has a molecular weight of approximately 17,000 daltons, and has different amino acid composition from AA, AL or AF type amyloid fibril proteins. Immunochemical analysis using antiserum raised against Nis-DAM and Nis-GAM proteins disclosed that positive reactions were obtained only with degraded amyloids of MCT and with sera from patients with MCT. In contrast, there were negative reactions for other type of amyloid proteins, sera from other type of amyloid, normal sera or urinary proteins. Therefore Nis-amyloid fibril protein has some antigenic determinants characteristic of MCT-related amyloid fibril protein.

摘要

从一名甲状腺髓样癌（MCT）患者中提取出一种独特的淀粉样纤维蛋白（Nis）。这种淀粉样蛋白的分子量约为17,000道尔顿，其氨基酸组成与AA、AL或AF型淀粉样纤维蛋白不同。使用针对Nis-DAM和Nis-GAM蛋白产生的抗血清进行免疫化学分析发现，仅与MCT的降解淀粉样蛋白以及MCT患者的血清产生阳性反应。相比之下，对其他类型的淀粉样蛋白、其他类型淀粉样变患者的血清、正常血清或尿蛋白均呈阴性反应。因此，Nis淀粉样纤维蛋白具有一些MCT相关淀粉样纤维蛋白特有的抗原决定簇。