Tang Wenmin, Pan Peng, Chen Tiantian, Wang Jian, Cui Xiangnan, Liu Wentao, Kan Liang
Department of Geriatrics，Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, PR China.
Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, PR China; School of Materials Science and Engineering, University of Science and Technology of China, Shenyang 110016, PR China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114199. doi: 10.1016/j.colsurfb.2024.114199. Epub 2024 Sep 2.
Bone defect has always been a difficult problem in clinical work. According to the current research results, tissue engineered scaffolds with a single function, structure, and composition are not sufficient to repair complex bone defects. In this work, a three-dimensional (3D) chitosan degradable composite scaffold loaded with zinc oxide (ZnO) was constructed, and the effect of ZnO content on scaffold performance and osteogenesis was explored. The 3D composite scaffold was prepared by freeze-drying technology. The microstructure, porosity, degradation performance, release performance, swelling performance, cytotoxicity, cell adhesion and osteogenic ability of ZnO nanoparticles and chitosan (ZnONPs/CS) composite scaffolds were measured. The results show that an appropriate amount of ZnO may be helpful to regulate the stability and degradation characteristics of the scaffold to a certain extent. Moreover, the composite scaffold could release ZnO into the simulated body fluid environment. The appropriate amount of ZnO helps to promote the proliferation, adhesion, and osteogenic differentiation of MC3T3-E1 cells. At a ZnO content of 3 wt%, both in vitro and vivo results showed relatively optimal biocompatibility and bioactivity of the scaffolds. This work could at least provide some positive insights for the selection of ZnO dosage, construction of chitosan-based 3D scaffolds, tissue engineering applications, and clinical treatment.
骨缺损一直是临床工作中的难题。根据目前的研究结果，单一功能、结构和组成的组织工程支架不足以修复复杂的骨缺损。在这项工作中，构建了一种负载氧化锌（ZnO）的三维（3D）壳聚糖可降解复合支架，并探讨了ZnO含量对支架性能和成骨作用的影响。通过冷冻干燥技术制备了3D复合支架。测定了氧化锌纳米颗粒与壳聚糖（ZnONPs/CS）复合支架的微观结构、孔隙率、降解性能、释放性能、溶胀性能、细胞毒性、细胞黏附性和成骨能力。结果表明，适量的ZnO可能在一定程度上有助于调节支架的稳定性和降解特性。此外，复合支架可将ZnO释放到模拟体液环境中。适量的ZnO有助于促进MC3T3-E1细胞的增殖、黏附和成骨分化。当ZnO含量为3 wt%时，体外和体内结果均显示支架具有相对最优的生物相容性和生物活性。这项工作至少可为ZnO用量的选择、基于壳聚糖的3D支架构建、组织工程应用及临床治疗提供一些积极的见解。
