Selmani Atiđa, Zeiringer Scarlett, Šarić Ankica, Stanković Anamarija, Učakar Aleksander, Vidmar Janja, Abram Anže, Njegić Džakula Branka, Kontrec Jasminka, Zore Anamarija, Bohinc Klemen, Roblegg Eva, Matijaković Mlinarić Nives
Pharmaceutical Technology and Biopharmacy, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 1, 8010 Graz, Austria.
Ruđer Bošković Institute, Bijenička Cesta 54, 10000 Zagreb, Croatia.
J Funct Biomater. 2025 Mar 19;16(3):108. doi: 10.3390/jfb16030108.
Loss of implant function is a common complication in orthopaedic and dental surgery. Among the primary causes of implant failure are peri-implant infections which often result in implant removal. This study demonstrates the development of a new antimicrobial titanium coating with ZnO nanoparticles of various sizes and morphologies immobilised in poly(allylamine hydrochloride) and alginate multilayers, combined with epitaxially grown vaterite crystals. The coated samples were characterised with various methods (FTIR, XRD, SEM) and surface properties were evaluated via water contact angle and surface charge measurements. Zinc ion release was quantified using ICP-MS. The antimicrobial efficacy of the coatings was tested against , , and while the biocompatibility was tested with preosteoblast cells (MC3T3-E1). Results demonstrated the successful preparation of a calcium carbonate/ZnO composite coating with epitaxially grown vaterite on titanium surfaces. The Zn ions released from ZnO nanoparticles dramatically influenced the morphology of vaterite where a new flower-like morphology was observed. The coated titanium surfaces exhibited robust antimicrobial activity, achieving over 90% microbial viability reduction for , , and . Importantly, the released Zn concentrations remained below the cytotoxicity limit for MC3T3-E1 cells, showing potential for safe and effective implant applications.
植入物功能丧失是骨科和牙科手术中常见的并发症。植入物失败的主要原因之一是种植体周围感染，这常常导致植入物被移除。本研究展示了一种新型抗菌钛涂层的开发，该涂层含有固定在聚（烯丙胺盐酸盐）和海藻酸盐多层膜中的各种尺寸和形态的ZnO纳米颗粒，并结合了外延生长的球霰石晶体。用各种方法（傅里叶变换红外光谱、X射线衍射、扫描电子显微镜）对涂层样品进行表征，并通过水接触角和表面电荷测量来评估表面性质。使用电感耦合等离子体质谱法定量锌离子释放。测试了涂层对 、 和 的抗菌效果，同时用前成骨细胞（MC3T3-E1）测试了生物相容性。结果表明成功制备了在钛表面外延生长有球霰石的碳酸钙/ZnO复合涂层。从ZnO纳米颗粒释放的锌离子极大地影响了球霰石的形态，观察到一种新的花状形态。涂覆的钛表面表现出强大的抗菌活性，对 、 和 实现了超过90%的微生物活力降低。重要的是，释放的锌浓度保持在MC3T3-E1细胞的细胞毒性极限以下，显示出在安全有效的植入物应用方面的潜力。
