Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Bioresour Technol. 2024 Nov;412:131422. doi: 10.1016/j.biortech.2024.131422. Epub 2024 Sep 2.
Rhodosporidium toruloides has emerged as a prominent candidate for producing single-cell oil from cost-effective feedstocks. In this study, the capability of R. toruloides to produce punicic acid (PuA), a representative plant unusual fatty acid, was investigated. The introduction of acyl lipid desaturase and conjugase (PgFADX) allowed R. toruloides to accumulate 3.7 % of total fatty acids as PuA. Delta-12 acyl lipid desaturase (PgFAD2) and diacylglycerol acyltransferase 2 were shown to benefit PuA production. The strain with PgFADX and PgFAD2 coexpression accumulated 12 % of its lipids as PuA from glucose, which translated into a PuA titer of 451.6 mg/L in shake flask condition. Utilizing wood hydrolysate as the feedstock, this strain produced 6.4 % PuA with a titer of 310 mg/L. Taken together, the results demonstrated that R. toruloides could serve as an ideal platform for the production of plant-derived high-value conjugated fatty acid using agricultural and forestry waste as feedstock.
红酵母已成为利用成本效益高的原料生产单细胞油脂的重要候选菌株。在本研究中，研究了红酵母生产代表植物独特脂肪酸——漆树酸（PuA）的能力。通过引入酰基辅酶 A 去饱和酶和共轭酶（PgFADX），红酵母能够将 3.7%的总脂肪酸积累为 PuA。Δ12 酰基辅酶 A 去饱和酶（PgFAD2）和二酰基甘油酰基转移酶 2 有利于 PuA 的生产。共表达 PgFADX 和 PgFAD2 的菌株能够从葡萄糖中积累 12%的脂质为 PuA，摇瓶条件下 PuA 的产量达到 451.6mg/L。利用木材水解物作为原料，该菌株生产了 6.4%的 PuA，产量为 310mg/L。综上所述，研究结果表明，红酵母可以作为一种理想的平台，利用农业和林业废物作为原料生产植物来源的高附加值共轭脂肪酸。
