Safro M G, Andreeva N S, Zhdanov A S
Mol Biol (Mosk). 1985 Mar-Apr;19(2):400-5.
The determination of the three dimensional structure of chymosin at 3 A resolution by molecular replacement method is described. The rotation functions for various aspartic proteases were calculated and combined results were used for the refinement of orientational parameters of chymosin molecules in the unit cell. The interpretation of Crowther-Blow translation function map with packing consideration enable to place correctly the molecules in the chymosin unit cell. Several difference Fourier syntheses for chymosin were calculated and differences between pepsin and chymosin structures were detected.
本文描述了通过分子置换法以3埃分辨率测定凝乳酶三维结构的过程。计算了各种天冬氨酸蛋白酶的旋转函数，并将组合结果用于精修晶胞中凝乳酶分子的取向参数。结合堆积考虑对克劳瑟-布洛平移函数图进行解读，能够正确地将分子置于凝乳酶晶胞中。计算了凝乳酶的几个差值傅里叶合成，并检测了胃蛋白酶和凝乳酶结构之间的差异。
