• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

掺入的溴脱氧尿苷对电离辐射和长波紫外线诱导CHO细胞染色体改变的影响。

Influence of incorporated bromodeoxyuridine on the induction of chromosomal alterations by ionizing radiation and long-wave UV in CHO cells.

作者信息

Zwanenburg T S, van Zeeland A A, Natarajan A T

出版信息

Mutat Res. 1985 Jun-Jul;150(1-2):283-92. doi: 10.1016/0027-5107(85)90124-1.

DOI:10.1016/0027-5107(85)90124-1
PMID:3923339
Abstract

Incorporation of BrdUrd into nuclear DNA sensitizes CHO cells (1) to the induction of chromosomal aberrations by X-rays and 0.5 MeV neutrons and (2) to induction of chromosomal aberrations and SCEs by lw-UV. We have attempted to establish a correlation between induced chromosomal alterations and induced single- or double-strand breaks in DNA. The data show that while DSBs correlate very well with X-ray-induced aberrations, no clear correlation could be established between lw-UV induced SSBs (including alkali-labile sites) and chromosomal alterations. In addition the effect of 3-aminobenzamide (3AB) on the induction of chromosomal aberrations and SCEs induced by lw-UV has been determined. It is shown that 3AB is without any effect when lw-UV-irradiated cells are posttreated with this inhibitor. The significance of these results is discussed.

摘要

将5-溴脱氧尿苷掺入核DNA中会使中国仓鼠卵巢细胞（1）对X射线和0.5兆电子伏特中子诱导的染色体畸变敏感，以及（2）对波长为254纳米的紫外线诱导的染色体畸变和姐妹染色单体交换敏感。我们试图建立DNA中诱导的染色体改变与诱导的单链或双链断裂之间的相关性。数据表明，虽然双链断裂与X射线诱导的畸变相关性很好，但在波长为254纳米的紫外线诱导的单链断裂（包括碱不稳定位点）与染色体改变之间未能建立明确的相关性。此外，还测定了3-氨基苯甲酰胺（3AB）对波长为254纳米的紫外线诱导的染色体畸变和姐妹染色单体交换的影响。结果表明，当用这种抑制剂对波长为254纳米的紫外线照射的细胞进行后处理时，3AB没有任何作用。讨论了这些结果的意义。

相似文献

1
Influence of incorporated bromodeoxyuridine on the induction of chromosomal alterations by ionizing radiation and long-wave UV in CHO cells.掺入的溴脱氧尿苷对电离辐射和长波紫外线诱导CHO细胞染色体改变的影响。
Mutat Res. 1985 Jun-Jul;150(1-2):283-92. doi: 10.1016/0027-5107(85)90124-1.
2
Influence of inhibitors of poly(ADP-ribose) polymerase on DNA repair, chromosomal alterations, and mutations.聚（ADP - 核糖）聚合酶抑制剂对DNA修复、染色体改变及突变的影响。
Princess Takamatsu Symp. 1983;13:227-42.
3
Cytological characterization of Chinese hamster ovary X-ray-sensitive mutant cells, xrs 5 and xrs 6. II. Induction of sister-chromatid exchanges and chromosomal aberrations by X-rays and UV-irradiation and their modulation by inhibitors of poly(ADP-ribose) synthetase and alpha-polymerase.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞学特征。II. X射线和紫外线照射诱导的姐妹染色单体交换和染色体畸变及其受聚（ADP - 核糖）合成酶和α - 聚合酶抑制剂的调节
Mutat Res. 1987 Mar;177(1):149-60. doi: 10.1016/0027-5107(87)90030-3.
4
Cytological characterization of repair-deficient CHO cell line 43-3B. I. Induction of chromosomal aberrations and sister-chromatid exchanges by UV and its modulation with 3-aminobenzamide.修复缺陷型中国仓鼠卵巢细胞系43 - 3B的细胞学特征。I.紫外线诱导染色体畸变和姐妹染色单体交换及其用3 - 氨基苯甲酰胺进行调节
Mutat Res. 1985 Apr;149(2):239-47. doi: 10.1016/0027-5107(85)90030-2.
5
3-Aminobenzamide sensitizes cultured Chinese hamster cells with bromodeoxyuridine-substituted DNA to genetic damage induced by long-wave UV.3-氨基苯甲酰胺使含有溴脱氧尿苷取代DNA的培养中国仓鼠细胞对长波紫外线诱导的遗传损伤敏感。
Mutat Res. 1985 Sep;144(1):41-9. doi: 10.1016/0165-7992(85)90122-8.
6
The contribution of DNA single-strand breaks to the formation of chromosome aberrations and SCEs.DNA单链断裂对染色体畸变和姐妹染色单体交换形成的作用。
Basic Life Sci. 1984;29 Pt A:229-44. doi: 10.1007/978-1-4684-4889-4_18.
7
Cytological characterization of Chinese hamster ovary X-ray-sensitive mutant cells xrs 5 and xrs 6. I. Induction of chromosomal aberrations by X-irradiation and its modulation with 3-aminobenzamide and caffeine.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞学特征。I. X射线照射诱导染色体畸变及其用3-氨基苯甲酰胺和咖啡因进行调节
Mutat Res. 1987 Mar;177(1):133-48. doi: 10.1016/0027-5107(87)90029-7.
8
Biochemical and cytogenetical characterization of Chinese hamster ovary X-ray-sensitive mutant cells xrs 5 and xrs 6. VI. The correlation between UV-induced DNA lesions and chromosomal aberrations, and their modulations with inhibitors of DNA repair synthesis.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的生化与细胞遗传学特征。VI.紫外线诱导的DNA损伤与染色体畸变之间的相关性，以及它们受DNA修复合成抑制剂的调节作用。
Mutat Res. 1990 Mar;235(2):129-35. doi: 10.1016/0921-8777(90)90066-e.
9
Cytogenetical characterization of Chinese hamster ovary X-ray-sensitive mutant cells, xrs 5 and xrs 6. IV. Study of chromosomal aberrations and sister-chromatid exchanges by restriction endonucleases and inhibitors of DNA topoisomerase II.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞遗传学特征。IV. 用限制性内切酶和DNA拓扑异构酶II抑制剂研究染色体畸变和姐妹染色单体交换
Mutat Res. 1989 Jun;212(2):137-48. doi: 10.1016/0027-5107(89)90064-x.
10
Use of the 5-bromodeoxyuridine-labelling technique for exploring mechanisms involved in the formation of chromosomal aberrations.使用5-溴脱氧尿苷标记技术探索染色体畸变形成所涉及的机制。
Mutat Res. 1980 Dec;73(2):307-17. doi: 10.1016/0027-5107(80)90196-7.

引用本文的文献

1
Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40.利用鸡B淋巴细胞系DT40进行同源DNA重组的反向遗传学研究。
Philos Trans R Soc Lond B Biol Sci. 2001 Jan 29;356(1405):111-7. doi: 10.1098/rstb.2000.0755.
2
X-irradiation of G1 CHO cells induces SCE which are both true and false in BrdU-substituted cells but only false in biotin-dUTP-substituted cells.对处于G1期的中国仓鼠卵巢细胞进行X射线照射会诱导姐妹染色单体交换，在掺入5-溴脱氧尿苷的细胞中，这种交换既有真性的也有假性的，但在掺入生物素脱氧三磷酸尿苷的细胞中只有假性的。
Chromosome Res. 1999;7(4):277-88. doi: 10.1023/a:1009226930759.