Zwanenburg T S, Csukás I, van Zeeland A A, Natarajan A T
Mutat Res. 1985 Sep;144(1):41-9. doi: 10.1016/0165-7992(85)90122-8.
Chinese hamster cells, containing BrdUrd-substituted DNA, were irradiated in the presence of 3-aminobenzamide with short-wave UV, long-wave UV or X-rays and analyzed for induced SCEs or chromosomal aberrations. The data presented in this paper show that when BrdUrd-substituted cells are irradiated with lw-UV in the presence of 3-aminobenzamide, genetic damage is increased. Biochemical analysis shows that the molecular weight of BrdUrd-substituted DNA is reduced by this treatment. The sensitization is due to the combined action of lw-UV, incorporated BrdUrd and 3-aminobenzamide, without any involvement of inhibition of poly(ADP-ribose) synthetase. No potentiation occurs when cells are irradiated with X-rays and genetic damage is decreased when cells are irradiated with UV light of 254 nm in the presence of 3AB. This decrease coincides with a reduction in the amount of induced pyrimidine dimers, detected as T4 endonuclease-sensitive sites in DNA.
含有BrdUrd取代DNA的中国仓鼠细胞，在3 - 氨基苯甲酰胺存在下，分别用短波紫外线、长波紫外线或X射线进行照射，然后分析诱导的姐妹染色单体交换（SCE）或染色体畸变情况。本文呈现的数据表明，当在3 - 氨基苯甲酰胺存在下用长波紫外线照射BrdUrd取代的细胞时，遗传损伤会增加。生化分析表明，这种处理会降低BrdUrd取代DNA的分子量。这种敏化作用是由于长波紫外线、掺入的BrdUrd和3 - 氨基苯甲酰胺的联合作用，而与聚（ADP - 核糖）合成酶的抑制无关。当用X射线照射细胞时不会出现增强作用，而当在3AB存在下用254nm紫外线照射细胞时，遗传损伤会减少。这种减少与作为DNA中T4内切核酸酶敏感位点检测到的诱导嘧啶二聚体数量的减少相一致。
