Aslan Coşkun, Eraslan Gökhan
Derinkuyu Emineana and Yaşar Ertaş Agriculture and Livestock Vocational School, Nevşehir Hacı Bektaş Veli University, Nevşehir, 50700, Turkey.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Erciyes University, Kayseri, 38039, Turkey.
Toxicol Res (Camb). 2024 Sep 3;13(5):tfae134. doi: 10.1093/toxres/tfae134. eCollection 2024 Oct.
The aim of the study was to investigate the effect of baicalin and baicalin-bovine serum albumin nanoparticles against bendiocarb exposure in rats.
Eighty male Wistar Albino rats aged 4-6 weeks were used. Corn oil (vehicle) alone was administered to the control group. To other groups, BSA-nanoparticle equivalent to that binding baicalin at a dose of 20 mg/kg.bw, 20 mg/kg.bw baicalin, baicalin-BSA nanoparticle equivalent to that binding baicalin at a dose of 20 mg/kg.bw, 4 mg/kg.bw bendiocarb, combination of 4 mg/kg.bw bendiocarb and 20 mg/kg.bw baicalin, combination of 4 mg/kg.bw bendiocarb and BSA-nanoparticle equivalent to that binding baicalin at a dose of 20 mg/kg.bw and combination of 4 mg/kg.bw bendiocarb and baicalin-BSA nanoparticle equivalent to that binding baicalin at a dose of 20 mg/kg.bw was administered to animals by oral gavage with vehicle for 21 days, after which organs (liver, kidney, brain, testes, heart and lung) and blood samples were collected. Blood/tissue oxidative stress (MDA, NO, GSH, SOD, CAT, GSH-Px, GR, GST, G6PD), serum biochemical (glucose, triglyceride, cholesterol, BUN, creatinine, uric acid, total protein, albumin, LDH, AST, ALT, ALP and ) and liver and kidney apoptotic/anti-apoptotic (caspase 3, 9, p53, Bcl-2 and Bax) parameters were evaluated. Body weights/organ weights and plasma/liver bendiocarb analyses were obtained.
While bendiocarb administered alone caused oxidative stress/tissue damage, baicalin and baicalin-BSA nanoparticle showed a mitigating effect. However, this effect was more pronounced in the baicalin-BSA nanoparticle group. BSA-nanoparticle alone did not have a significant effect in reversing the adverse effect caused by bendiocarb.
本研究旨在探讨黄芩苷及黄芩苷 - 牛血清白蛋白纳米粒对大鼠接触残杀威的影响。
选用80只4 - 6周龄的雄性Wistar白化大鼠。对照组仅给予玉米油（赋形剂）。给其他组动物经口灌胃给予相当于结合20 mg/kg体重黄芩苷的牛血清白蛋白纳米粒、20 mg/kg体重的黄芩苷、相当于结合20 mg/kg体重黄芩苷的黄芩苷 - 牛血清白蛋白纳米粒、4 mg/kg体重的残杀威、4 mg/kg体重残杀威与20 mg/kg体重黄芩苷的组合、4 mg/kg体重残杀威与相当于结合20 mg/kg体重黄芩苷的牛血清白蛋白纳米粒的组合以及4 mg/kg体重残杀威与相当于结合20 mg/kg体重黄芩苷的黄芩苷 - 牛血清白蛋白纳米粒的组合，同时给予赋形剂，持续21天，之后采集器官（肝脏、肾脏、脑、睾丸、心脏和肺）和血液样本。评估血液/组织氧化应激（丙二醛、一氧化氮、谷胱甘肽、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽 - S - 转移酶、葡萄糖 - 6 - 磷酸脱氢酶）、血清生化指标（葡萄糖、甘油三酯、胆固醇、血尿素氮、肌酐、尿酸、总蛋白、白蛋白、乳酸脱氢酶、天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶）以及肝脏和肾脏凋亡/抗凋亡指标（半胱天冬酶3、9、p53、Bcl - 2和Bax）。记录体重/器官重量并进行血浆/肝脏残杀威分析。
单独给予残杀威会引起氧化应激/组织损伤，黄芩苷和黄芩苷 - 牛血清白蛋白纳米粒显示出缓解作用。然而，这种作用在黄芩苷 - 牛血清白蛋白纳米粒组中更为明显。单独的牛血清白蛋白纳米粒在逆转残杀威所致的不良反应方面没有显著效果。
