Goya-Jorge Elizabeth, Jorge-Rodríguez María Elisa, Saucedo-Hernández Yanelis
Laboratory of Pharmaceutical Analysis, Department of Pharmacy, Faculty of Chemistry and Pharmacy, Universidad Central "Marta Abreu" de Las Villas, Santa Clara, Cuba.
Intestinal Regenerative Medicine Lab, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Nat Prod Res. 2024 Sep 5:1-7. doi: 10.1080/14786419.2024.2394097.
Cucurbitacins are triterpene bioactive constituents of natural products, particularly in the Cucurbitaceae plant family. The presence of cucurbitacins in seeds of the genus (pumpkin) has been only little studied. In this work, the content of cucurbitacins B, D, and E in seed oils from three cucurbits ( Duch, Linn, and Linn) was studied. An analytical method based on HPLC-DAD for the detection and quantification of these three cucurbitacins in seed oils was developed and validated according to ICH guidelines. The method showed good linearity, accuracy, and precision for the simultaneous quantification of cucurbitacins B, D, and E using seed oil as a reference. When applied to and seed oils, cucurbitacin B and D were quantified but to a lesser extent. This is the first report of a simple, repeatable, and reproducible analytical tool to identify cucurbitacins in oilseeds from spp.
葫芦素是天然产物中的三萜类生物活性成分，尤其存在于葫芦科植物家族中。葫芦属（南瓜）种子中葫芦素的存在情况研究较少。在本研究中，对三种葫芦科植物（西葫芦、南瓜和笋瓜）种子油中葫芦素B、D和E的含量进行了研究。开发了一种基于高效液相色谱 - 二极管阵列检测法（HPLC - DAD）的分析方法，用于检测和定量种子油中的这三种葫芦素，并根据国际协调会议（ICH）指南进行了验证。该方法在以种子油为参比同时定量葫芦素B、D和E时显示出良好的线性、准确性和精密度。当应用于西葫芦和笋瓜种子油时，可对葫芦素B和D进行定量，但含量较低。这是首次报道一种简单、可重复且可重现的分析工具，用于鉴定南瓜属油料种子中的葫芦素。
