Hussein Zain, Golovina Lidia A, Alaji Meera, Nour Moustapha A Y, Kolpashchikov Dmitry M, Komissarov Andrey B, El-Deeb Ahmed A
Laboratory of Nucleic Acid Nanotechnology, SCAMT Institute, ITMO University, 9 Lomonosova Str., 191002, St. Petersburg, Russian Federation.
Chemistry Department, University of Central Florida, Orlando, FL, 32816-2366, USA.
Chembiochem. 2024 Dec 2;25(23):e202400572. doi: 10.1002/cbic.202400572. Epub 2024 Oct 27.
We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5-fold, as compared to single-core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection.
我们介绍了一种多核DNA纳米机器（MDNM），它利用四种二元DNA酶进行核酸检测，无需预扩增步骤。与单核系统相比，这一创新显著降低了检测限（LOD），降幅超过5倍。这减少了所需的检测时间，从而突出了MDNM在推进核酸检测方面的潜力。
