Elsawy Noha Abdelhalim, Ibrahiem Aya Hanafy, Younis Gihan Abdellatif, Meheissen Marwa Ahmed, Abdel-Fattah Yousra Hisham
Department of Rheumatology, Rehabilitation and Physical Medicine, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Cartilage. 2024 Sep 5:19476035241276852. doi: 10.1177/19476035241276852.
To assess the relation between microbiome and lipopolysaccharide (LPS), in the blood and synovial fluid (SF) with femoral cartilage thickness (FCT) measured by ultrasound (US) in knee osteoarthritis (KOA) patients.
This cross-sectional study included 40 primary KOA patients recruited between September 2022 and June 2023. Age, gender, and body mass index (BMI) were recorded. Patients underwent full clinical examination, standing plain x-ray of the knee joint and knee US examination to measure medial, intercondylar, and lateral FCT. Microbiomes (specific bacterial phyla) were detected by real-time polymerase chain reaction and LPS levels were measured by enzyme-linked immunosorbent assay kit in the patients' serum and SF.
The patient's age ranged from 43 to 72 years. Most patients were females (72.5%), with a mean BMI of 35.8 ± 6.21 kg/m. The mean medial, intercondylar, and lateral FCT were less than cut-off values. All 40 (100%) patients showed positive bacterial deoxyribonucleic acid (16S ribosomal RNA) in both blood and SF samples. was the most abundant in patients' blood (48.49%) and SF (63.59%). The mean serum LPS level was significantly higher compared to mean SF LPS ( =4.702, < 0.001). There was a statistically significant negative correlation between lateral FCT and relative abundance in both patients' blood and SF.
Microbiome and LPS are present in the blood and SF of primary KOA patients. Microbiome () was associated with decreased lateral FCT. This might provide a potential link between both systemic and local microbiomes and cartilage affection in KOA patients.
评估膝骨关节炎（KOA）患者血液和滑液（SF）中的微生物群与脂多糖（LPS）之间的关系，以及通过超声（US）测量的股骨软骨厚度（FCT）。
这项横断面研究纳入了2022年9月至2023年6月招募的40例原发性KOA患者。记录年龄、性别和体重指数（BMI）。患者接受全面临床检查、膝关节站立位X线平片和膝关节超声检查，以测量内侧、髁间和外侧FCT。通过实时聚合酶链反应检测微生物群（特定细菌门），并使用酶联免疫吸附测定试剂盒测量患者血清和SF中的LPS水平。
患者年龄在43至72岁之间。大多数患者为女性（72.5%），平均BMI为35.8±6.21kg/m。内侧、髁间和外侧FCT的平均值均低于临界值。所有40例（100%）患者的血液和SF样本中均显示细菌脱氧核糖核酸（16S核糖体RNA）呈阳性。 在患者血液（48.49%）和SF（63.59%）中最为丰富。血清LPS平均水平显著高于SF LPS平均水平（ =4.702， < 0.001）。患者血液和SF中，外侧FCT与 相对丰度之间存在统计学显著负相关。
原发性KOA患者的血液和SF中存在微生物群和LPS。微生物群（ ）与外侧FCT降低有关。这可能为KOA患者全身和局部微生物群与软骨病变之间提供潜在联系。
