Institute of applied Ecology, Chinese Academy of Sciences, Shenyang, China.
University of Chinese Academy of Sciences, Beijing, China.
J Sci Food Agric. 2025 Jan 15;105(1):671-681. doi: 10.1002/jsfa.13863. Epub 2024 Sep 5.
The relationship between phosphorus (P) related enzymatic activity and organic P turnover remains unclear, particularly in the context of biochar application. Field experiments were conducted on Phaeozem and Luvisol soil types to investigate the effects of biochar application rates - 0 t ha (CK), 22.5 t ha (D), 67.5 t ha (D), and 112.5 t ha (D) - on soil organic fractions using P nuclear magnetic resonance (NMR) spectroscopy and relevant phosphatase activity.
The application of biochar increased the soil organic carbon (SOC), pyrophosphate (pyro), and orthophosphate (ortho) content, as well as the acid phosphomonoesterase (AcP), alkaline phosphomonoesterase (AlP), inorganic pyrophosphatase (IPP), and phosphodiesterase (PD) activities. Biochar application also increased soil organic P (OP), the sum of inorganic P forms (IP), ortho, monoesters, and myo-IHP contents, the pH value, AlP and PD activities in Phaeozem, but it significantly reduced diesters, polyphosphate (poly) contents, and IPP and AcP activities compared to those in Luvisol. Acid phosphomonoesterase and PD activities also showed an opposite trend in Luvisol. The structural equation model showed that the potential mechanism of organic P turnover in response to biochar application differed depending on the soil types, potentially influenced by P availability.
Overall, the findings of this study enhance the comprehension of the variation of P fractions and their availability in the context of biochar application for agricultural production in northeastern China. © 2024 Society of Chemical Industry.
磷（P）相关酶活性与有机磷转化之间的关系尚不清楚，尤其是在生物炭应用的情况下。本研究在黑钙土和淋溶土上进行了田间试验，以调查生物炭施用量（0 t ha（CK）、22.5 t ha（D）、67.5 t ha（D）和 112.5 t ha（D））对土壤有机磷的影响，采用磷核磁共振（NMR）光谱和相关磷酸酶活性来研究土壤有机磷。
生物炭的施用增加了土壤有机碳（SOC）、焦磷酸盐（pyro）和正磷酸盐（ortho）的含量，以及酸性磷酸单酯酶（AcP）、碱性磷酸单酯酶（AlP）、无机焦磷酸酶（IPP）和磷酸二酯酶（PD）的活性。生物炭的施用还增加了土壤有机磷（OP）、无机磷形态（IP）总和、正磷酸盐、单酯和肌醇六磷酸含量、pH 值、AlP 和 PD 活性在黑钙土中，但与淋溶土相比，它显著降低了二酯、多磷酸盐（poly）含量以及 IPP 和 AcP 活性。在淋溶土中，酸性磷酸单酯酶和 PD 活性也呈现出相反的趋势。结构方程模型表明，生物炭应用对有机磷转化的潜在机制因土壤类型而异，可能受磷供应的影响。
总的来说，本研究结果增强了对生物炭应用背景下 P 组分变化及其可利用性的理解，有助于促进中国东北地区农业生产中生物炭的应用。 © 2024 化学工业协会。
