State Key Laboratory of Integrated Management on Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
Jiangxi Provincial Key Laboratory of Conservation Biology, College of Forestry, Jiangxi Agricultural University, Nanchang, 330045, China.
Sci China Life Sci. 2024 Nov;67(11):2459-2470. doi: 10.1007/s11427-024-2679-3. Epub 2024 Aug 30.
Understanding the emergence and spread of antibiotic resistance genes (ARGs) in wildlife is critical for the health of humans and animals from a "One Health" perspective. The gut microbiota serve as a reservoir for ARGs; however, it remains poorly understood how environmental and host genetic factors influence ARGs by affecting the gut microbiota. To elucidate this, we analyzed whole-genome resequencing data from 79 individuals of Brandt's vole in two geographic locations with different antibiotics usage, together with metabolomic data and shotgun sequencing data. A high diversity of ARGs (851 subtypes) was observed in vole's gut, with a large variation in ARG composition between individuals from Xilingol and Hulunbuir in China. The diversity and composition of ARGs were strongly correlated with variations in gut microbiota community structure. Genome-wide association studies revealed that 803 loci were significantly associated (P<5.05×10) with 31 bacterial species, and bipartite networks identified 906 bacterial species-ARGs associations. Structural equation modeling analysis showed that host genetic factors, air temperature, and presence of pollutants (Bisphenol A) significantly affected gut microbiota community structure, which eventually regulated the diversity of ARGs. The present study advances our understanding of the complex host-environment interactions that underlie the spread of ARGs in the natural environments.
从“同一健康”的角度来看，了解抗生素耐药基因 (ARGs) 在野生动物中的出现和传播对于人类和动物的健康至关重要。肠道微生物群是 ARGs 的储存库；然而，环境和宿主遗传因素如何通过影响肠道微生物群来影响 ARGs 仍知之甚少。为了阐明这一点，我们分析了来自两个地理位置的 79 只布氏田鼠的全基因组重测序数据，这两个地理位置的抗生素使用情况不同，同时还分析了代谢组学数据和鸟枪法测序数据。在田鼠的肠道中观察到 ARG 的多样性很高（851 种亚型），来自中国锡林郭勒和呼伦贝尔的个体之间的 ARG 组成存在很大差异。ARG 的多样性和组成与肠道微生物群落结构的变化密切相关。全基因组关联研究表明，803 个基因座与 31 种细菌显著相关（P<5.05×10），二分网络确定了 906 种细菌-ARG 关联。结构方程模型分析表明，宿主遗传因素、空气温度和污染物（双酚 A）的存在显著影响了肠道微生物群落结构，最终调节了 ARG 的多样性。本研究增进了我们对复杂的宿主-环境相互作用的理解，这些相互作用是 ARGs 在自然环境中传播的基础。
