Department of Biotechnology and Food Engineering, Guangdong Technion-Israel Institute of Technology (GTIIT), Shantou, China.
Methods Mol Biol. 2025;2852:123-134. doi: 10.1007/978-1-0716-4100-2_8.
Properly using controllable atmospheric containers can facilitate investigations of the survival abilities and physiological states of key and emerging-foodborne pathogens under recreated applicable food processing environmental conditions. Notably, saturated salt solutions can efficiently control relative humidity in airtight containers. This chapter describes a practical experimental setup, with necessary prerequisites for exposing foodborne pathogens to simulated and relevant food processing environmental conditions. Subsequent analyses for studying cell physiology will also be suggested.
正确使用可控大气容器可以促进在再造适用的食品加工环境条件下对关键和新兴食源性病原体的生存能力和生理状态进行研究。值得注意的是,饱和盐溶液可以有效地控制密封容器中的相对湿度。本章描述了一种实用的实验设置,介绍了使食源性病原体暴露于模拟和相关食品加工环境条件下所需的必要前提条件。随后还将提出用于研究细胞生理学的分析建议。