School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong, 266237, China.
School of Life Sciences, Shandong University, Qingdao, Shandong, 266237, China.
Chemosphere. 2024 Sep;364:143263. doi: 10.1016/j.chemosphere.2024.143263. Epub 2024 Sep 3.
Swine wastewater application can introduce antibiotics, antibiotic resistance genes (ARGs) into environments. Herein, the full-scale transmission of antibiotics, ARGs and their potential carriers from an intensive swine feedlot to its surroundings were explored. Results showed that lincomycin and doxycycline hydrochloride were dominant antibiotics in this ecosystem. Lincomycin concentration were strongly associated with soil bacterial communities. According to the risk quotient (RQ), lincomycin was identified as posing higher ecological risk in aquatic environments. ARGs and mobile genetic elements (MGEs) abundance in wastewater were reduced after anaerobic treatment. Notably, ARGs composition of environmental samples were clustered into two groups based on if they were directly affected by the wastewater. However, there were no remarkable difference of ARGs abundance among environmental samples. The total abundance of ARGs was positively related to that of MGEs. Pathogens Escherichia coli and Enterococcus revealed strong connection with qnrS, tet and sul. Overall, this study highlights the importance of responsible antibiotics use in livestock production and appropriate treatment technology before agricultural application and discharge.
猪场废水的应用可能会将抗生素和抗生素抗性基因（ARGs）引入环境中。本研究探索了从集约化养猪场到周围环境中抗生素、ARGs 及其潜在载体的大规模传播。结果表明，林可霉素和盐酸多西环素是该生态系统中的主要抗生素。林可霉素浓度与土壤细菌群落密切相关。根据风险商数（RQ），林可霉素在水生环境中被认为具有更高的生态风险。厌氧处理后，废水中的 ARGs 和移动遗传元件（MGEs）丰度降低。值得注意的是，根据是否直接受到废水的影响，环境样本中的 ARGs 组成聚类为两组。然而，环境样本中 ARGs 的丰度没有明显差异。ARGs 的总丰度与 MGEs 的丰度呈正相关。病原菌大肠杆菌和肠球菌与 qnrS、tet 和 sul 表现出强烈的关联。总之，本研究强调了在农业应用和排放之前，在畜牧业生产中合理使用抗生素和适当的处理技术的重要性。
