National R&D Center for Freshwater Fish Processing, College of Chemistry and Materials, College of Life Science, School of Health, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
National R&D Center for Freshwater Fish Processing, College of Chemistry and Materials, College of Life Science, School of Health, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Int J Biol Macromol. 2024 Nov;279(Pt 4):135322. doi: 10.1016/j.ijbiomac.2024.135322. Epub 2024 Sep 3.
The study aimed to investigating the mechanisms of relieved intestinal barrier damage by dynamic high-pressure microfluidization assisted with galactooligosaccharide- glycated whey protein isolate. The modifications changed the multi-structure, and the modified whey protein isolate could promote the proliferation of IEC-6 cells and contributed to the restoration of LPS-induced occludin damage in IEC-6 cells. Also, it could repair cyclophosphamide-induced ileal villus rupture and crypt destruction in BALB/c mice, significantly altered the abundance of dominant bacteria, which were associated with propionic acid, butyric acid, isovaleric acid, and valeric acid. Ileum transcriptomics revealed that the modified whey protein isolate significantly regulate of the levels of Cstad, Cyp11a1, and Hs6st2 genes, relating to the increase of propionic acid, isovaleric acid, and valeric acid. In conclusion, galactooligosaccharide- modified whey protein isolate could regulate the level of Cstad, Cyp11a1 and Hs6st2 genes by altering the gut microbial structure and the level of SCFAs, thereby repairing the intestinal barrier.
本研究旨在探讨动态高压微射流辅助半乳糖低聚糖糖化乳清蛋白分离物缓解肠道屏障损伤的机制。修饰改变了多结构，修饰后的乳清蛋白分离物能够促进 IEC-6 细胞的增殖，并有助于恢复 LPS 诱导的 IEC-6 细胞中闭合蛋白的损伤。此外，它还可以修复环磷酰胺诱导的 BALB/c 小鼠回肠绒毛破裂和隐窝破坏，显著改变优势菌的丰度，与丙酸、丁酸、异丁酸和戊酸有关。回肠转录组学表明，修饰后的乳清蛋白分离物可显著调节 Cstad、 Cyp11a1 和 Hs6st2 基因的水平，与丙酸、异丁酸和戊酸的增加有关。总之，半乳糖低聚糖修饰的乳清蛋白分离物可以通过改变肠道微生物结构和 SCFAs 的水平来调节 Cstad、 Cyp11a1 和 Hs6st2 基因的水平，从而修复肠道屏障。
