Centre for Nanostructures and Advanced Materials, DSI-CSIR Nanotechnology Innovation Centre, Council for Scientific and Industrial Research, Pretoria 0001, South Africa.
Centre for Nanostructures and Advanced Materials, DSI-CSIR Nanotechnology Innovation Centre, Council for Scientific and Industrial Research, Pretoria 0001, South Africa.
J Virol Methods. 2024 Dec;330:115028. doi: 10.1016/j.jviromet.2024.115028. Epub 2024 Sep 3.
Addressing the need for accessible SARS-CoV-2 testing, carboxy-PEG 12-thiol functionalized gold nanoparticles conjugates were developed for rapid point-of-care (POC) detection against SARS-CoV-2 spike protein, pseudo-SARS-CoV-2, and authentic Beta SARS-CoV-2 virus particles. These conjugates leverage gold nanoparticles (AuNPs) as signal transducers, cross-linked to either angiotensin-converting enzyme 2 (ACE2) or SARS-CoV-2 spike protein receptor-binding domain (RBD) antibodies as bioreceptors and showed a distinct color shift from pink to blue. To assess their POC feasibility, the conjugates were integrated into facemasks and breathalyzers, wherein aerosolized SARS-CoV-2 antigens were successfully detected, producing a color change within 10 and 30 minutes for the breathalyzer and facemask prototypes, respectively. Furthermore, we explored quantitative analysis using varying concentrations of SARS-CoV-2 spike protein. Both conjugates demonstrated a linear relationship between blue color intensity and virus concentration, with linear ranges of 0.08-0.6 ng/mL and 0.04-0.5 ng/mL, respectively. Low limits of detection and quantification were also achieved. They exhibited specificity, responding solely to SARS-CoV-2 even in complex matrices containing diverse proteins, including the SARS-CoV-1 spike protein. Precision tests yielded coefficient of variations below 2 %, showcasing their remarkable reproducibility. This work presents a promising approach for rapid, sensitive, and specific POC detection of SARS-CoV-2 paving the way for improved pandemic response and management.
为满足对 SARS-CoV-2 检测的需求，开发了一种羧基-PEG12-巯基功能化的金纳米粒子缀合物，用于针对 SARS-CoV-2 刺突蛋白、假 SARS-CoV-2 和真实的 Beta SARS-CoV-2 病毒颗粒进行快速即时（POC）检测。这些缀合物利用金纳米粒子（AuNPs）作为信号转导器，交联到血管紧张素转换酶 2（ACE2）或 SARS-CoV-2 刺突蛋白受体结合域（RBD）抗体作为生物受体，显示出从粉红色到蓝色的明显颜色变化。为了评估它们在 POC 中的可行性，将缀合物集成到口罩和呼气分析仪中，其中成功检测到气溶胶化的 SARS-CoV-2 抗原，在呼气分析仪和口罩原型中分别在 10 分钟和 30 分钟内产生颜色变化。此外，我们还使用不同浓度的 SARS-CoV-2 刺突蛋白探索了定量分析。两种缀合物均表现出蓝色颜色强度与病毒浓度之间的线性关系，线性范围分别为 0.08-0.6ng/mL 和 0.04-0.5ng/mL。也实现了低检测限和定量限。它们表现出特异性，仅对 SARS-CoV-2 有反应，即使在含有多种蛋白质的复杂基质中，包括 SARS-CoV-1 刺突蛋白也是如此。精密测试的变异系数低于 2%，显示出其出色的重现性。这项工作提出了一种快速、敏感和特异性的 SARS-CoV-2 POC 检测的有前途的方法，为改善大流行应对和管理铺平了道路。
