Department of Pharmacy, Federal University of Sergipe, São Cristóvão, 49107-230 Sergipe, Brazil.
Department of Morphology, Federal University of Sergipe, São Cristóvão, 49107-230 Sergipe, Brazil.
J Appl Microbiol. 2024 Sep 2;135(9). doi: 10.1093/jambio/lxae232.
Evaluate the in vitro efficacy of the essential oils derived from Aloysia citrodora (Verbenaceae), Cymbopogon winterianus (Poaceae), and Ocimum gratissimum (Lamiaceae) against Acanthamoeba polyphaga trophozoites. Additionally, microemulsions formulated with these essential oils, along with their major components, were analyzed.
The prepared microemulsions were characterized using polarized light microscopy and rheological techniques. The amoebicidal activity was determined by measuring the inhibitory concentration (IC50). Flow cytometry was employed to detect membrane damage and alterations in trophozoites size. The results revealed transparent and thermodynamically stable microemulsions. The essential oil from O. gratissimum exhibited a lower IC50, with values of 280.66 and 47.28 µg ml-1 after 24 and 48 h, respectively. When microemulsions containing essential oils were tested, the IC50 values exhibited a reduction of over 80% after 24 h. Particularly, eugenol, a constituent of the O. gratissimum essential oil, displayed higher amoebicidal activity. The essential oils also caused damage to the cell membrane, resulting in the subsequent death of the trophozoites.
The EOs of A. citrodora, C. winterianus, and O. gratissimum and their microemulsions showed antiparasitic effect against A. polyphaga trophozoites, representing promising alternatives for the treatment of diseases caused by this protozoan.
评估柠檬香桃木（马鞭草科）、冬茅香（禾本科）和罗勒（唇形科）精油及其主要成分制成的微乳液对多形棘阿米巴滋养体的体外疗效。
用偏光显微镜和流变技术对制备的微乳液进行了表征。通过测定抑制浓度（IC50）来评估杀阿米巴活性。采用流式细胞术检测细胞膜损伤和滋养体大小的变化。结果表明，微乳液透明且热力学稳定。罗勒精油的 IC50 较低，分别为 24 小时和 48 小时后 280.66 和 47.28 µg ml-1。当测试含有精油的微乳液时，24 小时后 IC50 值降低了 80%以上。特别是罗勒精油的主要成分丁香酚具有更高的杀阿米巴活性。这些精油还会破坏细胞膜，导致滋养体死亡。
柠檬香桃木、冬茅香和罗勒精油及其微乳液对多形棘阿米巴滋养体具有抗寄生虫作用，为治疗这种原生动物引起的疾病提供了有前途的选择。
