College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Plant Cell Rep. 2024 Sep 5;43(9):228. doi: 10.1007/s00299-024-03315-7.
Rice OsSPL11 activates the expression of GW5L through binding to its promoter and positively regulates grain size. Grain size (GS) is an important determinant of grain weight and yield potential in cereal. Here, we report the functional analysis of OsSPL11 in grain length (GL), grain width (GW), and 1000-grain weight (TGW). OsSPL11 mutant plants, osspl11 lines, exhibited a decrease in GL, GW, and TGW, and OsSPL11-OE lines showed an increase in GL and TGW. Expression analysis revealed that OsSPL11 was located in the nucleus and highly expressed in spikelet hull and young development grains, consistent with its function in determining GS. Further analysis confirmed that OsSPL11 directly activates the expression of GW5L to regulate GS, meanwhile OsSPL11 expression is negatively regulated by OsGBP3. Taken together, our findings demonstrate that OsSPL11 could be a key regulator of affecting GS during the spikelet hull development and facilitate the process of improving grain yield by GS modification in rice.
水稻 OsSPL11 通过与 GW5L 启动子结合激活其表达，从而正向调控粒长。粒长是谷类作物粒重和产量潜力的重要决定因素。在此，我们报告了 OsSPL11 在粒长（GL）、粒宽（GW）和千粒重（TGW）方面的功能分析。OsSPL11 突变体植物 osspl11 系表现出 GL、GW 和 TGW 的降低，而 OsSPL11-OE 系表现出 GL 和 TGW 的增加。表达分析表明，OsSPL11 位于细胞核中，在小穗颖壳和幼嫩发育籽粒中高度表达，与它在决定粒长方面的功能一致。进一步的分析证实，OsSPL11 直接激活 GW5L 的表达以调节 GS，同时 OsSPL11 的表达受到 OsGBP3 的负调控。综上所述，我们的研究结果表明，OsSPL11 可能是影响小穗颖壳发育过程中 GS 的关键调节因子，并有助于通过改良 GS 提高水稻的粒重。
