Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Udupi, Karnataka, 576104, India.
Manipal Center for Infectious Diseases (MAC ID), Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
AAPS PharmSciTech. 2024 Sep 5;25(7):203. doi: 10.1208/s12249-024-02909-4.
Normal skin is the first line of defense in the human body. A burn injury makes the skin susceptible to bacterial infection, thereby delaying wound healing and ultimately leading to sepsis. The chances of biofilm formation are high in burn wounds due to the presence of avascular necrotic tissue. The most common pathogen to cause burn infection and biofilm is Pseudomonas aeruginosa. The purpose of this study was to create a microemulsion (ME) formulation for topical application to treat bacterial burn infection. In the present study, tea tree oil was used as the oil phase, Tween 80 and transcutol were used as surfactants, and water served as the aqueous phase. Pseudo ternary phase diagrams were used to determine the design space. The ranges of components as suggested by the design were chosen, optimization of the microemulsion was performed, and in vitro drug release was assessed. Based on the characterization studies performed, it was found that the microemulsion were formulated properly, and the particle size obtained was within the desired microemulsion range of 10 to 300 nm. The I release study showed that the microemulsion followed an immediate release profile. The formulation was further tested based on its ability to inhibit biofilm formation and bacterial growth. The prepared microemulsion was capable of inhibiting biofilm formation.
正常皮肤是人体的第一道防线。烧伤会使皮肤容易受到细菌感染，从而延迟伤口愈合，最终导致败血症。由于存在无血管性坏死组织，烧伤创面形成生物膜的可能性很高。引起烧伤感染和生物膜的最常见病原体是铜绿假单胞菌。本研究旨在创建一种用于局部应用的微乳剂（ME）制剂来治疗细菌烧伤感染。在本研究中，茶树油用作油相，吐温 80 和 Transcutol 用作表面活性剂，水用作水相。使用伪三元相图来确定设计空间。根据设计建议选择了组件的范围，对微乳剂进行了优化，并评估了体外药物释放。根据进行的表征研究，发现微乳剂的配方正确，获得的粒径在所需的微乳剂范围内，为 10 至 300nm。I 释放研究表明，微乳剂遵循即刻释放曲线。根据其抑制生物膜形成和细菌生长的能力进一步测试了该制剂。所制备的微乳剂能够抑制生物膜的形成。
