Zabihi Mohammad Reza, Akhoondian Mohammad, Tohidian Mobina, Karkhah Samad, Ghorbani Vajargah Pooyan, Mazhari Seyed Amirhossein, Farhadi Bahar, Farzan Ramyar
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran.
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University.
Ann Med Surg (Lond). 2024 Jul 17;86(9):5162-5169. doi: 10.1097/MS9.0000000000002240. eCollection 2024 Sep.
The present study employs bioinformatics tools to identify shared upregulated genes between chemical burns and gastric cancer.
Gene Expression Omnibus (GEO) retrieved gene sets for this investigation. GSEs with value less than 0.05 and LOG fold change (FC) greater than 1 were valid and upregulated. Gastric cancer and chemical burn common elevated genes were found using Venn diagram online tools. In the second stage, the "string" visualized gastric cancer elevated genes network, and non-coding RNAs were deleted, and "interaction" greater than 1 was examined to choose important gene nodes. Next, they explored the String gene-interaction network for common genes. To determine the most interacting genes, Gephi (V 0.9.7) used "betweenness centrality" greater than "0" to evaluate the twenty-gene network. TISIDB and drug banks provide gene-related medications.
In the present study, two genes, including ALOX5AP and SERPINB2, were obtained, with the highest centrality among chemical burns and gastric cancer shared genes. Additionally, the current study presented five drugs, including Urokinase, Tenecteplase, DG031, AM103, and Fiboflapon, which can have predicted effects on gastric cancer following chemical burns.
According to current in-silicon analyses, ALOX5AP and SERPINB2 are linked genetic keys between gastric chemical burn and cancer. Considering that burn is an environmental factor that leads to the upregulation of the two genes thus, the chemical burn can be related to the incidence of gastric cancer.
本研究采用生物信息学工具来识别化学灼伤和胃癌之间共同上调的基因。
基因表达综合数据库（GEO）检索用于本研究的基因集。P值小于0.05且对数变化倍数（FC）大于1的基因集有效且上调。使用在线维恩图工具找出胃癌和化学灼伤共同上调的基因。在第二阶段，“String”工具可视化胃癌上调基因网络，删除非编码RNA，并检查“相互作用”大于1的情况以选择重要基因节点。接下来，他们探索共同基因的String基因相互作用网络。为确定相互作用最强的基因，Gephi（V 0.9.7）使用“中介中心性”大于“0”来评估二十基因网络。TISIDB和药物库提供与基因相关的药物。
在本研究中，获得了两个基因，即ALOX5AP和SERPINB2，它们在化学灼伤和胃癌共享基因中具有最高的中心性。此外，本研究还展示了五种药物，包括尿激酶、替奈普酶、DG031、AM103和纤维黄酮，它们对化学灼伤后的胃癌可能有预测作用。
根据目前的硅基分析，ALOX5AP和SERPINB2是胃化学灼伤和癌症之间的关键遗传联系。鉴于灼伤是导致这两个基因上调的环境因素，因此化学灼伤可能与胃癌的发病有关。
