Machado-Pereira Nicolas A M M, do Nascimento Patrícia S, de Freitas Gabriel R, Bobinski Franciane, do Espírito Santo Caroline C, Ilha Jocemar
Núcleo de Pesquisa em Lesão da Medula Espinal (NULEME), Departamento de Fisioterapia, Centro de Ciências da Saúde e do Esporte (CEFID), Universidade do Estado de Santa Catarina (UDESC), Florianópolis, SC, Brasil.
Departamento de Fisiologia e Farmacologia, Centro de Ciências da Saúde (CCS), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brasil.
Rev Bras Ortop (Sao Paulo). 2024 Sep 4;59(4):e526-e531. doi: 10.1055/s-0044-1787767. eCollection 2024 Aug.
To analyze the muscle trophism and expression of interleukin-6 in the biceps brachii muscle of rats with incomplete cervical spinal cord injury treated with neuromuscular electrical stimulation (NMES). Adult rats underwent C5-C7 spinal cord hemisection and a 5-week NMES protocol. Trophism of the biceps brachii was assessed using muscle weight/body weight ratio and histological analysis. Interleukin-6 expression from biceps brachii was measured using the enzyme-linked immunosorbent assay technique. Preservation of the biceps brachii muscle trophism was found in the NMES treated group, along with prevention of the reduction of interleukin-6 levels. Spinal cord injury causes muscle atrophy and decreases interleukin-6 levels. These alterations are partially prevented by NMES. The results suggest a possible NMES action mechanism and underscore the clinical use of this therapeutic tool.
分析经神经肌肉电刺激（NMES）治疗的不完全性颈脊髓损伤大鼠肱二头肌的肌肉营养状况及白细胞介素-6的表达。成年大鼠行C5-C7脊髓半横断术，并接受为期5周的NMES方案。通过肌肉重量/体重比和组织学分析评估肱二头肌的营养状况。采用酶联免疫吸附测定技术测量肱二头肌中白细胞介素-6的表达。在NMES治疗组中发现肱二头肌肌肉营养状况得以保留，同时白细胞介素-6水平降低得到预防。脊髓损伤会导致肌肉萎缩并降低白细胞介素-6水平。这些改变可通过NMES部分预防。结果提示了NMES可能的作用机制，并强调了这种治疗工具的临床应用。
