Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Vasc Med. 2024 Dec;29(6):640-652. doi: 10.1177/1358863X241268893. Epub 2024 Sep 6.
Mitochondrial abnormalities exist in lower-extremity peripheral artery disease (PAD), yet the association of the ankle-brachial index (ABI) with mitochondrial respiration in gastrocnemius muscle is unknown. The association of gastrocnemius mitochondrial respiration with 6-minute walk distance in PAD is unknown. This objective of this study was to describe associations of the ABI with mitochondrial respiratory function in gastrocnemius muscle biopsies and associations of gastrocnemius mitochondrial respirometry with 6-minute walk distance in people with and without PAD.
People with (ABI ⩽ 0.90) and without (ABI 1.00-1.40) PAD were enrolled. ABI and 6-minute walk distance were measured. Mitochondrial function of permeabilized myofibers from gastrocnemius biopsies was measured with high-resolution respirometry.
A total of 30 people with PAD (71.7 years, mean ABI: 0.64) and 68 without PAD (71.8 years, ABI: 1.17) participated. In non-PAD participants, higher ABI values were associated significantly with better mitochondrial respiration (Pearson correlation for maximal oxidative phosphorylation P: +0.29, = 0.016). In PAD, the ABI correlated negatively and not significantly with mitochondrial respiration (Pearson correlation for P: -0.17, = 0.38). In people without PAD, better mitochondrial respiration was associated with better 6-minute walk distance (Pearson correlation: +0.51, < 0.001), but this association was not present in PAD (Pearson correlation: +0.10, = 0.59).
Major differences exist between people with and without PAD in the association of gastrocnemius mitochondrial respiration with ABI and 6-minute walk distance. Among people without PAD, ABI and walking performance were positively associated with mitochondrial respiratory function. These associations were not observed in PAD.
下肢外周动脉疾病（PAD）存在线粒体异常，但踝臂指数（ABI）与腓肠肌线粒体呼吸的关系尚不清楚。腓肠肌线粒体呼吸与 PAD 患者 6 分钟步行距离的关系尚不清楚。本研究的目的是描述 ABI 与腓肠肌活检中线粒体呼吸功能的关系，以及腓肠肌线粒体呼吸测定与 PAD 患者和无 PAD 患者 6 分钟步行距离的关系。
纳入有（ABI ⩽ 0.90）和无（ABI 1.00-1.40）PAD 的患者。测量 ABI 和 6 分钟步行距离。使用高分辨率呼吸测定法测量腓肠肌活检中肌纤维的线粒体功能。
共有 30 名 PAD 患者（71.7 岁，平均 ABI：0.64）和 68 名无 PAD 患者（71.8 岁，ABI：1.17）参与了研究。在无 PAD 参与者中，较高的 ABI 值与更好的线粒体呼吸显著相关（最大氧化磷酸化 Pearson 相关性：+0.29，P = 0.016）。在 PAD 中，ABI 与线粒体呼吸呈负相关，但无统计学意义（Pearson 相关性：-0.17，P = 0.38）。在无 PAD 的人群中，更好的线粒体呼吸与更好的 6 分钟步行距离相关（Pearson 相关性：+0.51，P < 0.001），但在 PAD 中则没有相关性（Pearson 相关性：+0.10，P = 0.59）。
有和无 PAD 的人群中，ABI 和 6 分钟步行距离与腓肠肌线粒体呼吸的关系存在显著差异。在无 PAD 的人群中，ABI 和运动表现与线粒体呼吸功能呈正相关。这些相关性在 PAD 中则没有观察到。
