Anand-Srivastava M B, Johnson R A, Picard S, Cantin M
Biochem Biophys Res Commun. 1985 May 31;129(1):171-8. doi: 10.1016/0006-291x(85)91418-4.
The effect of ninhibin, a sperm factor extracted from bovine sperm, was studied on adenylate cyclase from rat aorta. Ninhibin treatment of the membranes activated adenylate cyclase in a concentration dependent manner. The maximal activation (approximately equal to 4-fold) was obtained at 2micrograms ninhibin and at 10 min of treatment at 37 degrees C. On the other hand, in untreated control membranes, ninhibin at 2 micrograms could stimulate adenylate cyclase by about 50-60% only. In addition, ninhibin potentiated the guanine nucleotide-, isoproterenol- and forskolin (FSK)-stimulated adenylate cyclase activities and also attenuated the GTP gamma s and atrial natriuretic factor (ANF)-mediated inhibition of enzyme activities. Furthermore the inhibition of isoproterenol- and FSK-stimulated adenylate cyclase activities by ANF was also abolished by ninhibin. These data indicate that ninhibin which has been suggested to inactivate or inhibit Ni-guanine nucleotide regulatory protein can also attenuate the ANF-receptor mediated inhibition of adenylate cyclase in rat aorta suggesting an involvement of Ni-guanine nucleotide regulatory protein in the coupling of ANF receptors to adenylate cyclase.
研究了从牛精子中提取的精子因子抑制素对大鼠主动脉腺苷酸环化酶的作用。用抑制素处理细胞膜可使腺苷酸环化酶呈浓度依赖性激活。在37℃处理10分钟、抑制素浓度为2微克时可获得最大激活（约为4倍）。另一方面，在未处理的对照细胞膜中，2微克抑制素仅能刺激腺苷酸环化酶约50 - 60%。此外，抑制素增强了鸟嘌呤核苷酸、异丙肾上腺素和福斯高林（FSK）刺激的腺苷酸环化酶活性，并且减弱了GTPγS和心房利钠因子（ANF）介导的酶活性抑制。此外，抑制素也消除了ANF对异丙肾上腺素和FSK刺激的腺苷酸环化酶活性的抑制作用。这些数据表明，曾被认为可使Ni - 鸟嘌呤核苷酸调节蛋白失活或抑制其活性的抑制素，也能减弱大鼠主动脉中ANF受体介导的腺苷酸环化酶抑制作用，提示Ni - 鸟嘌呤核苷酸调节蛋白参与了ANF受体与腺苷酸环化酶的偶联。
