Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Methods Mol Biol. 2025;2848:59-71. doi: 10.1007/978-1-0716-4087-6_4.
Glaucoma is one of the leading causes of irreversible blindness. Stem cell therapy has shown promise in the treatment of primary open-angle glaucoma in animal models. Stem cell-free therapy using stem cell-derived trophic factors might be in demand in patients with high-risk conditions or religious restrictions. In this chapter, we describe methods for trabecular meshwork stem cell (TMSC) cultivation, secretome harvesting, and protein isolation, as well as assays to ensure the health of TMSC post-secretome harvesting and for secretome periocular injection into mice for therapeutic purposes.
青光眼是导致不可逆性失明的主要原因之一。干细胞疗法在动物模型中对原发性开角型青光眼的治疗显示出了前景。对于高危人群或有宗教限制的患者，使用干细胞衍生的营养因子的无干细胞疗法可能会有需求。在这一章中，我们描述了小梁网干细胞（TMSC）培养、分泌组收获和蛋白质分离的方法，以及确保 TMSC 分泌组收获后和用于治疗目的的分泌组眶周注射的健康的检测方法。
