Department of Emergency and Critical Care Center, Renmin Hospital, Hubei University of Medicine, Hubei, China.
Hum Exp Toxicol. 2024 Jan-Dec;43:9603271241282584. doi: 10.1177/09603271241282584.
Environmental factors such as noise and music can significantly impact physiological responses, including inflammation. This study explored how environmental factors like noise and music affect lipopolysaccharide (LPS)-induced inflammation, with a focus on systemic and organ-specific responses.
24 Wistar rats were divided into four groups ( = 6 per group): Control group, LPS group, noise-exposed group, and music-exposed group. All rats, except for the Control group, received 10 mg/kg LPS intraperitoneally. The rats in the noise-exposed group were exposed to 95 dB noise, and the music-exposed group listened to Mozart's K. 448 music (65-75 dB) for 1 h daily over 7 days. An enzyme-linked immunosorbent assay was utilized to detect the levels of inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), in serum and tissues (lung, liver, and kidney). Western blot examined the phosphorylation levels of nuclear factor-κB (NF-κB) p65 in organ tissues.
Compared with the Control group, LPS-induced sepsis rats displayed a significant increase in the levels of TNF-α and IL-1β in serum, lung, liver, and kidney tissues, as well as a remarkable elevation in the p-NF-κB p65 protein expression in lung, liver, and kidney tissues. Noise exposure further amplified these inflammatory markers, while music exposure reduced them in LPS-induced sepsis rats.
Noise exposure exacerbates inflammation by activating the NF-κB pathway, leading to the up-regulation of inflammatory markers during sepsis. On the contrary, music exposure inhibits NF-κB signaling, indicating a potential therapeutic effect in reducing inflammation.
环境因素，如噪音和音乐，可显著影响生理反应，包括炎症。本研究探讨了环境因素，如噪音和音乐如何影响脂多糖（LPS）诱导的炎症，重点关注全身和器官特异性反应。
24 只 Wistar 大鼠随机分为 4 组（每组 n = 6）：对照组、LPS 组、噪声暴露组和音乐暴露组。除对照组外，所有大鼠均腹腔内注射 10 mg/kg LPS。噪声暴露组大鼠暴露于 95 dB 噪声中，音乐暴露组大鼠每天听莫扎特的 K. 448 音乐（65-75 dB）1 小时，持续 7 天。采用酶联免疫吸附试验检测血清和组织（肺、肝、肾）中炎症细胞因子，包括肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的水平。Western blot 检测组织（肺、肝、肾）中核因子-κB（NF-κB）p65 的磷酸化水平。
与对照组相比，LPS 诱导的脓毒症大鼠血清、肺、肝、肾组织中 TNF-α和 IL-1β水平显著升高，肺、肝、肾组织中 p-NF-κB p65 蛋白表达明显升高。噪声暴露进一步放大了这些炎症标志物，而音乐暴露则降低了 LPS 诱导的脓毒症大鼠的这些标志物。
噪声暴露通过激活 NF-κB 通路加重炎症，导致脓毒症期间炎症标志物上调。相反，音乐暴露抑制 NF-κB 信号，表明其在减轻炎症方面具有潜在的治疗作用。
