XN Biotechnologies, LLC, St. Louis, Missouri 63110, United States.
Mallinckrodt Institute of Radiology, Washington University in St. Louis, St. Louis, Missouri 63110, United States.
ACS Appl Bio Mater. 2024 Oct 21;7(10):6392-6397. doi: 10.1021/acsabm.4c01027. Epub 2024 Sep 6.
In this work, we develop recombinant human cationic ferritin (rHCF) as a contrast agent to detect glomeruli in the kidney using positron emission tomography (PET). We first expressed recombinant human ferritin (rHF) in and then functionalized and radiolabeled it with Copper-64 (Cu) to form Cu-rHCF. Intravenously injected Cu-rHCF bound to kidney glomeruli and was detected by PET. A subchronic toxicity study after an intravenous injection of rHCF revealed no significant toxicity. The development of rHCF is an important step toward the potential clinical translation of CF to detect the nephron number in humans.
在这项工作中，我们开发了重组人阳离子铁蛋白（rHCF）作为对比剂，通过正电子发射断层扫描（PET）检测肾脏中的肾小球。我们首先在大肠杆菌中表达重组人铁蛋白（rHF），然后用铜-64（Cu）对其进行功能化和放射性标记，形成 Cu-rHCF。静脉注射的 Cu-rHCF 与肾脏肾小球结合，并用 PET 检测。静脉注射 rHCF 后的亚慢性毒性研究表明没有明显的毒性。rHCF 的开发是 CF 向临床转化以检测人类肾单位数量的重要一步。
