State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China; Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, PR China.
State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China.
J Chromatogr A. 2024 Oct 25;1735:465282. doi: 10.1016/j.chroma.2024.465282. Epub 2024 Aug 17.
In this study, lincomycin was successfully purified by macroporous adsorption resin column chromatography using the HZ3 resin. The optimal separation parameters were set as follows: the column bed height was 33 cm, sample loading capacity was 48 mg/mL and flow rate of loading was 1 mL/min. A mixture of 0.02 mol/L of NaHPO∙12HO (pH = 8.5, adjusted using HPO) and acetone (80:20, v/v) was used as the eluent. The elution flow rate was maintained at 3 mL/min. Under these parameters, the purity of lincomycin calculated using the standard curve was 99.00 %, with the yield being 97.84 %. This enrichment and separation method of lincomycin is highly regarded owing to its remarkable efficiency and straightforward operation. Thus, the proposed method for the separation and purification of lincomycin holds considerable promise for pharmaceutical applications.
在这项研究中，林可霉素通过大孔吸附树脂柱色谱法使用 HZ3 树脂成功地被纯化。最佳分离参数设置如下：柱床高度为 33 厘米，样品装载容量为 48mg/mL，装载流速为 1mL/min。使用 0.02mol/L 的 NaHPO∙12HO（pH=8.5，使用 HPO 调节）和丙酮（80:20，v/v）的混合物作为洗脱液。洗脱流速保持在 3mL/min。在这些参数下，使用标准曲线计算得到的林可霉素的纯度为 99.00%，收率为 97.84%。由于其高效和操作简便，这种林可霉素的富集和分离方法受到高度重视。因此，所提出的林可霉素分离和纯化方法在药物应用方面具有很大的前景。
