Liang Yuan-Yuan, Lu Zhou, Liu Hong-Wei, Huang Qi, Zheng Xue-Ting, Li Xiao-An, Zhou Yan
NHC Key Laboratory of Nuclear Technology Medical Transformation, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Mianyang 621000, Sichuan, China.
Institute of Fluid Physics, CAEP, Mianyang 621000, China.
Bioelectrochemistry. 2025 Feb;161:108803. doi: 10.1016/j.bioelechem.2024.108803. Epub 2024 Aug 31.
Nanosecond Pulsed Electric Fields (nsPEFs) treatment has demonstrated anti-tumor effects on various cancer cell lines. However, the use of this treatment in pancreatic cancer is limited. This study demonstrated that nsPEFs treatment effectively suppressed the proliferation and metastasis of pancreatic cancer cells, while also inducing DNA damage. Meanwhile, animal experiments have shown that nsPEFs effectively suppressed the growth of pancreatic cancer, even in cases where the tumor volume exceeded 500-600 mm at the initiation of treatment. Notably, a single treatment session was found to significantly inhibit tumor growth, while also showing no adverse effects on the main organs of the mice. RNA sequencing and bioinformatics revealed that seven key genes (CDK1, CENPA, UBE2C, CCNB2, PLK1, CCNA2, and CCNB14) were significantly correlated with the overall survival rate of patients with pancreatic cancer. Through the application of the competing endogenous RNA (ceRNA) hypothesis, two miRNAs (has-let-7b-5p and hsa-miR-193b-3p) and four lncRNAs (MIR4435-2HG, ZNF436-AS1, LINC01089, and MIR4435-2HG) were identified as significantly impacting the overall survival of pancreatic cancer patients. We have effectively developed an mRNA-miRNA-lncRNA network that has the potential to stimulate further investigation into the underlying mechanisms of nsPEFs on pancreatic cancer.
纳秒脉冲电场（nsPEFs）治疗已在多种癌细胞系中显示出抗肿瘤作用。然而，这种治疗方法在胰腺癌中的应用有限。本研究表明，nsPEFs治疗可有效抑制胰腺癌细胞的增殖和转移，同时诱导DNA损伤。与此同时，动物实验表明，即使在治疗开始时肿瘤体积超过500 - 600立方毫米的情况下，nsPEFs也能有效抑制胰腺癌的生长。值得注意的是，单次治疗就可显著抑制肿瘤生长，且对小鼠的主要器官无不良影响。RNA测序和生物信息学分析显示，七个关键基因（CDK1、CENPA、UBE2C、CCNB2、PLK1、CCNA2和CCNB14）与胰腺癌患者的总生存率显著相关。通过应用竞争性内源RNA（ceRNA）假说，鉴定出两个miRNA（has-let-7b-5p和hsa-miR-193b-3p）和四个lncRNA（MIR4435-2HG、ZNF436-AS1、LINC01089和MIR4435-2HG）对胰腺癌患者的总生存有显著影响。我们有效地构建了一个mRNA-miRNA-lncRNA网络，这可能会激发对nsPEFs作用于胰腺癌潜在机制的进一步研究。
