Department of Chemistry, Indian Institute of Technology Madras, Chennai 600 036, India.
Department of Chemistry, Indian Institute of Technology Madras, Chennai 600 036, India.
Int J Biol Macromol. 2024 Nov;279(Pt 3):135336. doi: 10.1016/j.ijbiomac.2024.135336. Epub 2024 Sep 4.
The preparation of soilless medium for the growth of seeds based on macroporous scaffolds of chitosan-glutaric acid and urea (CHGAUR) is reported. The CHGAUR scaffold was prepared by the hydrothermal reaction of chitosan with GA in presence of urea. The product was purified using Soxhlet extraction with methanol. CHGAUR was composed of chitosan with a small weight fraction of glutaric acid and urea, which were present in the salt form as confirmed by TGA and FT IR spectroscopy. This suggested that CH is most likely to be physically crosslinked in the scaffold through GAUR links. SEM images confirmed the porous structure of material. Water absorption studies suggested that CHGAUR could absorb ∼4100-5700 % water and could be varied by varying the CH: UR ratio used in the preparation. The percentage porosity of CHGAUR did not vary much with urea content suggesting that significant variation in the micropore structure was not obtained by varying the urea content in the preparation. Mechanical tests revealed that material was soft and flexible and able to retain its original shape even after removing the load. CHGAUR, in purified state, was studied for the germination and seedling growth. Among the seeds investigated, pigeon pea showed the best result.
报道了一种基于壳聚糖-戊二酸和尿素（CHGAUR）大孔支架的无土培养基的制备。CHGAUR 支架是通过壳聚糖与 GA 在尿素存在下的水热反应制备的。产物通过甲醇索氏提取进行纯化。TGA 和 FTIR 光谱证实，CHGAUR 由带有少量戊二酸和尿素的壳聚糖组成，它们以盐的形式存在，这表明 CH 很可能通过 GAUR 键在支架中发生物理交联。SEM 图像证实了材料的多孔结构。吸水研究表明，CHGAUR 可以吸收约 4100-5700%的水，并且可以通过改变制备中使用的 CH:UR 比来改变。CHGAUR 的孔隙率百分比变化不大，表明通过改变制备中尿素的含量，微孔结构没有明显变化。机械测试表明，该材料柔软且有弹性，即使在去除负载后也能保持其原始形状。纯化后的 CHGAUR 用于研究发芽和幼苗生长。在所研究的种子中，鸽豆表现出最好的结果。
