Department of Organic Chemistry, Faculty of Chemistry, Shahid Beheshti University, Tehran, G.C, Iran.
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Bioprocess Biosyst Eng. 2024 Dec;47(12):2027-2041. doi: 10.1007/s00449-024-03083-4. Epub 2024 Sep 6.
Encapsulating the enzyme in metal-organic frameworks (MOFs) is a convenient method to prepare MOF-enzyme biocomposite. In this study, Candida antarctica lipase B (CAL-B) was chosen to immobilize in Cu-BTC MOF under ultrasound irradiation. CAL-B was immobilized in Cu-BTC under ultrasound at 21 kHz and 11.4 W/cm and incubation. 98% of CAL-B was immobilized in Cu-BTC with 99 U/mg activity (threefold more active than the free CAL-B). The prepared biocomposite was characterized using FT-IR, XRD, TGA, SEM, EDX, and BET. The thermal and solvent stability of CAL-B@Cu-BTC was investigated. It was found that at a temperature of 55 ℃, CAL-B@Cu-BTC maintains its activity even after 2 h of incubation. Furthermore, in the presence of 20% and 50% concentrations of MeCN, THF, and DMF, CAL-B@Cu-BTC was found to have an activity of over 80%. A prepared biocatalyst was used to synthesize 1,3,4,5-tetrasubstituted pyrazole derivatives (50-75%) in a one-pot vessel, by adding phenyl hydrazine hydrochlorides, benzaldehydes, and dimethyl acetylenedicarboxylate.
将酶封装在金属有机骨架（MOFs）中是制备 MOF-酶生物复合材料的一种方便方法。在这项研究中，选择南极假丝酵母脂肪酶 B（CAL-B）在超声辐射下固定在 Cu-BTC MOF 中。CAL-B 在超声 21 kHz 和 11.4 W/cm 以及孵育条件下固定在 Cu-BTC 中。98%的 CAL-B 以 99 U/mg 的活性（比游离 CAL-B 高三倍）固定在 Cu-BTC 中。使用 FT-IR、XRD、TGA、SEM、EDX 和 BET 对制备的生物复合材料进行了表征。研究了 CAL-B@Cu-BTC 的热稳定性和溶剂稳定性。结果发现，在 55℃的温度下，CAL-B@Cu-BTC 在孵育 2 小时后仍保持其活性。此外，在存在 20%和 50%浓度的 MeCN、THF 和 DMF 时，CAL-B@Cu-BTC 的活性超过 80%。制备的生物催化剂用于在一锅容器中通过添加苯肼盐酸盐、苯甲醛和二甲基丙炔酸二甲酯来合成 1,3,4,5-四取代吡唑衍生物（50-75%）。
