Department of Chemistry, KU Leuven, Leuven, Belgium.
Department of Nuclear Medicine, University Hospital RWTH Aachen University, Aachen, Germany.
Nat Methods. 2024 Oct;21(10):1801-1805. doi: 10.1038/s41592-024-02383-7. Epub 2024 Sep 6.
We present a way to encode more information in fluorescence imaging by splitting the original point spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the 'Circulator', an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.
我们提出了一种通过分裂原始点扩散函数 (PSF) 来编码更多荧光成像信息的方法，该方法提供了宽带操作，并且与其他 PSF 工程模式和现有分析工具兼容。我们使用“循环器”演示了该方法，该循环器将荧光团发射带编码到 PSF 中，从而能够使用基本上整个视场同时进行多色超分辨率和单分子显微镜。
