Precision Immunology Institute, Translational Transplant Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L Levy Place, Box 1243, New York, NY, 10029, USA.
Renal Unit, Department of Medicine, University Hospital of Verona, Verona, Italy.
BMC Immunol. 2024 Sep 6;25(1):58. doi: 10.1186/s12865-024-00650-y.
Systemic Lupus Erythematosus (SLE) has a strong genetic susceptibility, but little is known about the impact of diet on disease severity. The Western diet is typically deficient in magnesium (Mg), and given the immunomodulatory effects of Mg, we hypothesized that the low Mg intake increases disease risk and that increasing Mg intake would reduce severity of murine lupus. Here, we placed 12-week old MRL/lpr female lupus mice on a normal (Mg500) or a high (Mg2800) Mg diet for 9 weeks. Urine and blood were collected during the study for quantification of urinary albumin, BUN, anti-dsDNA antibodies, and immune phenotyping.
MRL/lpr lupus mice on high Mg2800 diet had significantly fewer skin lesions and less severe skin histology score, and reduced levels of pathogenic anti-dsDNA antibodies, compared with the Mg500 group (143.8±75.0 vs. 47.4±36.2 × 10U/ml; P < 0.05). The high Mg2800 group had a nearly two-fold increase in the percentage of CD4FOXP3 Treg cells compared to controls (19.9±5.4 vs. 11.4±5.5%; P < 0.05). Treg percentages inversely correlated with the concentration of anti-dsDNA. None of the mice developed arthritis during the observation period and there were no significant differences in weight, proteinuria, BUN or kidney histology.
In conclusion, oral supplementation of Mg has a protective effect in a murine lupus model and may represent an inexpensive and safe adjuvant in the treatment of SLsE.
系统性红斑狼疮（SLE）具有很强的遗传易感性，但人们对饮食对疾病严重程度的影响知之甚少。西方饮食通常缺乏镁（Mg），鉴于 Mg 的免疫调节作用，我们假设低 Mg 摄入会增加疾病风险，而增加 Mg 摄入会降低小鼠狼疮的严重程度。在这里，我们将 12 周龄的 MRL/lpr 雌性狼疮小鼠置于正常（Mg500）或高（Mg2800）Mg 饮食中 9 周。在研究过程中收集尿液和血液，用于定量测定尿白蛋白、BUN、抗 dsDNA 抗体和免疫表型。
与 Mg500 组相比，高 Mg2800 饮食组的 MRL/lpr 狼疮小鼠皮肤损伤明显减少，皮肤组织学评分较低，致病性抗 dsDNA 抗体水平降低（143.8±75.0 与 47.4±36.2 × 10U/ml；P<0.05）。与对照组相比，高 Mg2800 组的 CD4FOXP3 Treg 细胞百分比增加近两倍（19.9±5.4 与 11.4±5.5%；P<0.05）。Treg 百分比与抗 dsDNA 的浓度呈反比。在观察期间，没有一只小鼠发生关节炎，体重、蛋白尿、BUN 或肾脏组织学均无显著差异。
总之，口服补充 Mg 对小鼠狼疮模型具有保护作用，可能是治疗 SLE 的一种廉价且安全的辅助手段。
