Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department, School of Chemistry and Environment, Yunnan Minzu University, Kunming, 650500, PR China.
Chemosphere. 2024 Sep;364:143278. doi: 10.1016/j.chemosphere.2024.143278. Epub 2024 Sep 5.
Utilizing metal luminescence enhancement to design fluorescent probes is a very sensible strategy. Herein, a fluorescent probe based on europium (III)-functionalized silver nanoparticles-conjugated homocysteine (AgNPs-Hcy-Eu) was proposed for the selective and sensitive detection of tetracycline (TC). In this probe, Eu(III) was employed as the detection signal unit for TC, while AgNPs-Hcy was used as the ligand of fluorescence enhancement. When TC exists, it can bind to Eu immobilized in AgNPs-Hcy, leading to an enhanced fluorescence signal from Eu through energy transfer. Under optimal conditions, the fluorescence intensity of AgNPs-Hcy-Eu increased linearly with increasing TC concentration in the range of 0.1-30 μM (R = 0.9964). The fluorescent probe own fluorescence enhancement, paving the way for sensitive detection with a low detection limit of 0.083 μM. It also has good selectivity for common antibiotics and anions. This work can be applied to the determination of TC in tap water and milk with recoveries of 94-98.5%. We expect AgNPs-Hcy-Eu to have potential applications in environmental testing and food safety.
利用金属发光增强来设计荧光探针是一种非常明智的策略。本文提出了一种基于铕（III）功能化的银纳米粒子-结合同型半胱氨酸（AgNPs-Hcy-Eu）的荧光探针，用于选择性和灵敏地检测四环素（TC）。在该探针中，Eu(III)被用作 TC 的检测信号单元，而 AgNPs-Hcy 则用作荧光增强的配体。当 TC 存在时，它可以与固定在 AgNPs-Hcy 中的 Eu 结合，导致通过能量转移从 Eu 发出增强的荧光信号。在最佳条件下，AgNPs-Hcy-Eu 的荧光强度随 TC 浓度在 0.1-30 μM 范围内呈线性增加（R=0.9964）。该荧光探针具有荧光增强功能，为具有低检测限（0.083 μM）的灵敏检测铺平了道路。它对常见的抗生素和阴离子也具有良好的选择性。这项工作可应用于自来水中和牛奶中 TC 的测定，回收率为 94-98.5%。我们期望 AgNPs-Hcy-Eu 在环境测试和食品安全方面具有潜在的应用。
