线粒体基因组的结构分析揭示了檀香的复杂分支构型。

Structural analysis of the mitochondrial genome of Santalum album reveals a complex branched configuration.

机构信息

Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, 418008 Huaihua, Hunan, China.; College of Biological and Food Engineering, Huaihua University, 418008 Huaihua, Hunan, China.

Center for Biodiversity Conservation and Utilization, Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, 400715 Beibei, Chongqing, China.

出版信息

Genomics. 2024 Sep;116(5):110935. doi: 10.1016/j.ygeno.2024.110935. Epub 2024 Sep 5.

DOI:10.1016/j.ygeno.2024.110935

PMID:39243912

Abstract

BACKGROUND

Santalum album L. is an evergreen tree which is mainly distributes throughout tropical and temperate regions. And it has a great medicinal and economic value.

RESULTS

In this study, the complete mitochondrial genome of S. album were assembled and annotated, which could be descried by a complex branched structure consisting of three contigs. The lengths of these three contigs are 165,122 bp, 93,430 bp and 92,491 bp. We annotated 34 genes coding for proteins (PCGs), 26 tRNA genes, and 4 rRNA genes. The analysis of repeated elements shows that there are 89 SSRs and 242 pairs of dispersed repeats in S. album mitochondrial genome. Also we found 20 MTPTs among the chloroplast and mitochondria. The 20 MTPTs sequences span a combined length of 22,353 bp, making up 15.52 % of the plastome, 6.37 % of the mitochondrial genome. Additionally, by using the Deepred-mt tool, we found 628 RNA editing sites in 34 PCGs. Moreover, significant genomic rearrangement is observed between S. album and its associated mitochondrial genomes. Finally, based on mitochondrial genome PCGs, we deduced the phylogenetic ties between S. album and other angiosperms.

CONCLUSIONS

We reported the mitochondrial genome from Santalales for the first time, which provides a crucial genetic resource for our study of the evolution of mitochondrial genome.

摘要

背景

檀香科檀香属植物檀香为常绿乔木，主要分布于热带和温带地区，具有较高的药用和经济价值。

结果

本研究完成了檀香的线粒体基因组测序、组装和注释，其线粒体基因组由 3 个不同的大片段组成，分别为 165122bp、93430bp 和 92491bp。共注释到 34 个蛋白编码基因（PCGs）、26 个 tRNA 基因和 4 个 rRNA 基因。通过对重复序列的分析，在檀香的线粒体基因组中发现了 89 个 SSR 位点和 242 对散布重复序列。此外，在叶绿体和线粒体基因组中还发现了 20 个线粒体转移肽（MTPT）。这 20 个 MTPT 序列总长 22353bp，占叶绿体基因组的 15.52%，占线粒体基因组的 6.37%。同时，利用 Deepred-mt 工具，在 34 个 PCGs 中共发现 628 个 RNA 编辑位点。檀香与其近缘线粒体基因组之间存在显著的基因组重排。最后，基于线粒体基因组 PCGs 推断了檀香与其他被子植物的系统发育关系。