从头组装和两种品种的线粒体基因组的比较分析。

De Novo Assembly and Comparative Analysis of Mitochondrial Genomes of Two Varieties.

机构信息

National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, China.

Agricultural and Animal Husbandry Industry Development Research Institute, Guangxi University, Nanning 530004, China.

出版信息

Int J Mol Sci. 2024 May 22;25(11):5656. doi: 10.3390/ijms25115656.

is a species with important medicinal value and a complex genetic background. In this study, we sequenced and assembled the mitochondrial (mt) genomes of two varieties of . The mt genome lengths of var. and var. were 457,390 bp and 456,731 bp, respectively. Both mitogenomes showed a multi-branched structure consisting of two circular molecules, with 56 genes annotated, comprising 33 protein-coding genes, 18 tRNA genes ( and are multi-copy genes), and 3 rRNA genes. Then, 207 pairs of long repeats and 96 simple sequence repeats (SSRs) were detected in the mt genomes of and 484 potential RNA-editing sites were found across the 33 mitochondrial protein-coding genes of each variety. Additionally, a syntenic sequence analysis showed a high collinearity between the two mt genomes. This work is the first to analyze the mt genomes of It can provide information that can be used to analyze the structure of mt genomes of higher plants and provide a foundation for future comparative genomic studies and evolutionary biology research in related species.

是一种具有重要药用价值和复杂遗传背景的物种。在本研究中，我们对两种的线粒体（mt）基因组进行了测序和组装。 var. 和 var. 的 mt 基因组长度分别为 457,390 bp 和 456,731 bp。这两个 mt 基因组均呈现出具有两个圆形分子的多分支结构，共注释了 56 个基因，包括 33 个蛋白质编码基因、18 个 tRNA 基因（和是多拷贝基因）和 3 个 rRNA 基因。然后，在和的 mt 基因组中检测到 207 对长重复序列和 96 个简单序列重复（SSR），并在每个品种的 33 个线粒体蛋白质编码基因中发现了 484 个潜在的 RNA 编辑位点。此外，共线性分析显示两个 mt 基因组之间具有高度的同源性。这项工作首次分析了的 mt 基因组。它可以提供用于分析高等植物 mt 基因组结构的信息，并为相关物种的未来比较基因组研究和进化生物学研究提供基础。