College of Food science, Northeast Agricultural university, Harbin, Heilongjiang 150030, China.
College of Food science, Northeast Agricultural university, Harbin, Heilongjiang 150030, China.
Int J Biol Macromol. 2024 Nov;279(Pt 3):135372. doi: 10.1016/j.ijbiomac.2024.135372. Epub 2024 Sep 5.
In this experiment, the co-constructed O/W emulsions of different soy protein hydrolysates (SPHs) and gum arabic (GA) were investigated. SPHs were prepared by hydrolyzing soy protein isolate (SPI) using different enzymes, and investigated the effects of enzyme types and hydrolysis time on the physicochemical properties of SPHs. Moreover, SPI/GA and SPHs/GA were prepared and used as hydrophilic emulsifiers to construct O/W emulsions. The results showed that the optimal hydrolysis times for bromelain, pepsin and trypsin were 2 h (BSPH2), 3 h (PSPH3) and 3 h (TSPH3), respectively. Compared with SPI/GA emulsions, SPHs/GA emulsions had smaller particle size, more negative charge, higher interfacial adsorbed protein, and more stable emulsion systems. During the digestion process, SPHs/GA emulsions were effective in realizing the release of bioactives. In conclusion, enzymatic hydrolysis can be an effective modification technique, and SPHs/GA can be used as an effective emulsifier for the emulsion system.
在这项实验中，研究了不同大豆蛋白水解物（SPH）和阿拉伯胶（GA）的共构建 O/W 乳液。通过使用不同的酶水解大豆蛋白分离物（SPI）制备 SPH，并研究了酶类型和水解时间对 SPH 理化性质的影响。此外，还制备了 SPI/GA 和 SPHs/GA 并用作亲水性乳化剂来构建 O/W 乳液。结果表明，菠萝蛋白酶、胃蛋白酶和胰蛋白酶的最佳水解时间分别为 2 h（BSPH2）、3 h（PSPH3）和 3 h（TSPH3）。与 SPI/GA 乳液相比，SPHs/GA 乳液具有更小的粒径、更负的电荷、更高的界面吸附蛋白和更稳定的乳液体系。在消化过程中，SPHs/GA 乳液有效地实现了生物活性物质的释放。总之，酶解可以是一种有效的修饰技术，并且 SPHs/GA 可以用作乳液体系的有效乳化剂。
