College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, China.
College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, China; Key Laboratory of Molecular Designing and Green Conversions, Huaqiao University, Xiamen, 361021, China; Xiamen Key Laboratory of Optoelectronic Materials and Advanced Manufacturing, Huaqiao University, Xiamen, 361021, China.
Anal Chim Acta. 2024 Oct 9;1325:343114. doi: 10.1016/j.aca.2024.343114. Epub 2024 Aug 15.
Temperature sensing is commonly used in point-of-care (POC) detection technologies, yet the portability and convenience of use are frequently compromised by the complexity of thermosensitive processes and signal transduction. Especially, multi-step target recognition reactions and temperature measurement in the reaction vessel present challenges in terms of stability and integration of detection devices. To further combine photothermal reaction and signal readout in one assay, these two processes enable to be integrated into miniaturized microfluidic chips, thereby facilitating photothermal sensing and achieving a simple visual temperature sensing as POC detection.
A copper ion (Cu)-catalyzed photothermal sensing system integrated onto a microfluidic distance-based analytical device (μDAD), enabling the visual, portable, and sensitive quantitative detection of multiple targets, including ascorbic acid, glutathione, and alkaline phosphatase (ALP). The polydopamine nanoparticles (PDA NPs) were synthesized by the regulation of free Cu through redox or coordination reactions, facilitating the transduction of distinct photothermal response signals and providing the versatile Cu-responsive sensing systems. Promoted by integration with a photothermal μDAD, the system combines PDA's photothermal responsiveness and thermosensitive gas production of ammonium bicarbonate for improved sensitivity of ALP detection, reaching the detection limit of 9.1 mU/L. The system has successfully achieved on-chip detection of ALP with superior anti-interference capability and recoveries ranging from 96.8 % to 104.7 %, alongside relative standard deviations below 8.0 %.
The μDAD design accommodated both the photothermal reaction of PDA NPs and thermosensitive gas production reaction, achieving the rapid sensing of visual distance signals. The μDAD-based Cu-catalyzed photothermal sensing system holds substantial potential for applications in biochemical analysis and clinical diagnostics, underscored by the versatile Cu regulation mechanism for a broad spectrum of biomarkers.
温度感应通常用于即时检测（POC）技术中，但热敏过程和信号转导的复杂性经常影响其便携性和使用便利性。特别是，在反应容器中进行多步目标识别反应和温度测量，在检测设备的稳定性和集成方面带来了挑战。为了进一步将光热反应和信号读取结合在一个检测中，这两个过程可以集成到微型化微流控芯片中，从而实现光热感应，并实现简单的视觉温度感应作为 POC 检测。
将铜离子（Cu）催化的光热感应系统集成到基于微流体距离的分析设备（μDAD）上，实现了对多种目标的可视化、便携和敏感的定量检测，包括抗坏血酸、谷胱甘肽和碱性磷酸酶（ALP）。通过氧化还原或配位反应调节游离 Cu 合成聚多巴胺纳米粒子（PDA NPs），促进了不同的光热响应信号的转导，并提供了多功能的 Cu 响应感应系统。通过与光热 μDAD 的集成，该系统结合了 PDA 的光热响应性和碳酸氢铵的热敏气体产生，提高了 ALP 检测的灵敏度，检测限达到 9.1 mU/L。该系统成功实现了芯片上的 ALP 检测，具有优异的抗干扰能力和回收率在 96.8%至 104.7%之间，相对标准偏差低于 8.0%。
μDAD 设计同时容纳了 PDA NPs 的光热反应和热敏气体产生反应，实现了可视化距离信号的快速感应。基于 μDAD 的 Cu 催化光热感应系统具有广泛的生物标志物的多功能 Cu 调节机制，在生化分析和临床诊断中具有很大的应用潜力。
