Takizawa H, Sato S, Kitajima H, Konishi S, Iwata K, Hayashi Y
Carcinogenesis. 1985 Jun;6(6):921-3. doi: 10.1093/carcin/6.6.921.
Mouse skin melanomas were induced in two stage skin carcinogenesis with 7,12-dimethylbenz[a]anthracene as initiator and croton oil as promoter. After approximately 25 weeks of promotion, small black macules of the skin were observed in C57BL, CDF1 and BDF1 mice, and progressively grew with time. Macules less than 2 mm in diameter were localized mostly in the lower portion of the dermis and, histologically, these lesions were consistent with the diagnosis of melanocytoma and were composed of polygonal to round cells loaded with large numbers of melanin granules. The cells were closely packed forming well-demarcated cell-nests with occasional columnar arrangement. In the macules over 2 mm in diameter, the cells were closely packed to form irregularly bordered cell-nests, showing invasive growth into the surrounding tissues. The lesions were diagnosed as malignant melanomas. The incidence of benign and/or malignant melanoma differed among strains: 80% in BDF1, 70% in CDF1, 30% in C57BL/6 and 0% in DBA/2 mice. One example of tumor induced in a CDF1 mouse was transplantable to homologous CDF1 mice.
以7,12-二甲基苯并[a]蒽为启动剂、巴豆油为促癌剂，通过两阶段皮肤致癌法诱导小鼠皮肤黑色素瘤。在促癌约25周后，在C57BL、CDF1和BDF1小鼠的皮肤上观察到黑色小结节，并随时间逐渐增大。直径小于2 mm的小结节大多位于真皮下部，组织学上，这些病变符合黑素细胞瘤的诊断，由充满大量黑色素颗粒的多边形至圆形细胞组成。细胞紧密排列，形成界限清晰的细胞巢，偶尔呈柱状排列。在直径超过2 mm的小结节中，细胞紧密排列形成边界不规则的细胞巢，显示出向周围组织的浸润性生长。这些病变被诊断为恶性黑色素瘤。良性和/或恶性黑色素瘤的发生率在不同品系中有所不同：BDF1小鼠为80%，CDF1小鼠为70%，C57BL/6小鼠为30%，DBA/2小鼠为0%。在一只CDF1小鼠中诱导产生的肿瘤可移植到同基因的CDF1小鼠体内。
