• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

雌、雄红松个体对 UV-B 辐射的响应存在差异，涉及改变微生物群落组成与宿主次生代谢之间的相互作用。

Sex-specific responses of Taxus mairei to UV-B radiation involved altering the interactions between the microbiota assembly and host secondary metabolism.

机构信息

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.

Kharkiv Institute, Hangzhou Normal University, Hangzhou, 311121, China.

出版信息

Microbiome. 2024 Sep 7;12(1):165. doi: 10.1186/s40168-024-01882-1.

DOI:10.1186/s40168-024-01882-1
PMID:39244575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11380788/
Abstract

BACKGROUND

To adapt to constantly changing environments, ancient gymnosperms have coevolved with diverse endophytic fungi that are essential for the fitness and adaptability of the plant host. However, the effect of sex on plant-endophyte interactions in response to environmental stressors remains unknown. RNA-seq integrated with ITS analysis was applied to reveal the potential mechanisms underlying the sex-specific responses of Taxus mairei to ultraviolet (UV)-B radiation.

RESULTS

Enrichment analysis suggested that sex influenced the expression of several genes related to the oxidation-reduction system, which might play potential roles in sex-mediated responses to UV-B radiations. ITS-seq analysis clarified the effects of UV-B radiation and sex on the composition of endophytic fungal communities. Sex influenced various secondary metabolic pathways, thereby providing chemicals for T. mairei host to produce attractants and/or inhibitors to filter microbial taxa. Analysis of fungal biomarkers suggested that UV-B radiation reduced the effect of sex on fungal communities. Moreover, Guignardia isolate #1 was purified to investigate the role of endophytic fungi in sex-mediated responses to UV-B radiation. Inoculation with spores produced by isolate #1 significantly altered various oxidation-reduction systems of the host by regulating the expression of APX2, GST7 NCED1, ZE1, CS1, and CM1.

CONCLUSION

These results revealed the roles of endophytic fungi in sex-mediated responses to UV-B radiation and provided novel insights into the sex-specific responses of Taxus trees to environmental stressors. Video Abstract.

摘要

背景

为了适应不断变化的环境，古老的裸子植物与多样化的内生真菌共同进化，内生真菌对植物宿主的健康和适应性至关重要。然而，性别对植物-内生菌相互作用响应环境胁迫的影响尚不清楚。本研究采用 RNA-seq 与 ITS 分析相结合的方法，揭示了南方红豆杉对紫外线（UV）-B 辐射的性别特异性响应的潜在机制。

结果

富集分析表明，性别影响与氧化还原系统相关的几个基因的表达，这些基因可能在性别介导的对 UV-B 辐射的响应中发挥潜在作用。ITS-seq 分析阐明了 UV-B 辐射和性别对内生真菌群落组成的影响。性别影响各种次生代谢途径，从而为宿主南方红豆杉产生引诱剂和/或抑制剂提供化学物质，以筛选微生物类群。真菌生物标志物分析表明，UV-B 辐射降低了性别对真菌群落的影响。此外，还纯化了 Guignardia 分离株#1 以研究内生真菌在性别介导的对 UV-B 辐射的响应中的作用。接种分离株#1 的孢子显著改变了宿主的各种氧化还原系统，通过调节 APX2、GST7 NCED1、ZE1、CS1 和 CM1 的表达来实现。

结论

这些结果揭示了内生真菌在性别介导的对 UV-B 辐射的响应中的作用，并为 Taxus 树对环境胁迫的性别特异性响应提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/7deae197cd6f/40168_2024_1882_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/0f18c8484fc3/40168_2024_1882_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/dc6972334092/40168_2024_1882_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/13546f0cdca5/40168_2024_1882_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/530650049ad8/40168_2024_1882_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/8c44bc4a3da1/40168_2024_1882_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/b2f3167df9a7/40168_2024_1882_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/3b4f250a5da7/40168_2024_1882_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/7deae197cd6f/40168_2024_1882_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/0f18c8484fc3/40168_2024_1882_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/dc6972334092/40168_2024_1882_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/13546f0cdca5/40168_2024_1882_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/530650049ad8/40168_2024_1882_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/8c44bc4a3da1/40168_2024_1882_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/b2f3167df9a7/40168_2024_1882_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/3b4f250a5da7/40168_2024_1882_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cde6/11380788/7deae197cd6f/40168_2024_1882_Fig8_HTML.jpg

相似文献

1
Sex-specific responses of Taxus mairei to UV-B radiation involved altering the interactions between the microbiota assembly and host secondary metabolism.雌、雄红松个体对 UV-B 辐射的响应存在差异，涉及改变微生物群落组成与宿主次生代谢之间的相互作用。
Microbiome. 2024 Sep 7;12(1):165. doi: 10.1186/s40168-024-01882-1.
2
Fungal endophytes of Taxus species and regulatory effect of two strains on taxol synthesis.紫杉属植物内生真菌及其两菌株对紫杉醇合成的调控作用。
BMC Microbiol. 2024 Aug 3;24(1):291. doi: 10.1186/s12866-024-03445-8.
3
Diversity of endophytic fungi and screening of fungal paclitaxel producer from Anglojap yew, Taxus x media.从英国红豆杉（Taxus x media）中内生真菌的多样性及筛选产紫杉醇真菌
BMC Microbiol. 2013 Mar 28;13:71. doi: 10.1186/1471-2180-13-71.
4
Geographic and tissue influences on endophytic fungal communities of Taxus chinensis var. mairei in China.中国南方红豆杉内生真菌群落的地理和组织影响。
Curr Microbiol. 2013 Jan;66(1):40-8. doi: 10.1007/s00284-012-0235-z. Epub 2012 Sep 29.
5
An endophytic fungus efficiently producing paclitaxel isolated from Taxus wallichiana var. mairei.从南方红豆杉中分离得到的一种高效产生紫杉醇的内生真菌。
Medicine (Baltimore). 2017 Jul;96(27):e7406. doi: 10.1097/MD.0000000000007406.
6
[Diversity and community structure of endophytic fungi from Taxus chinensis var. mairei].[南方红豆杉内生真菌的多样性与群落结构]
Ying Yong Sheng Tai Xue Bao. 2014 Jul;25(7):2071-8.
7
[Root microstructure and distribution of the endophytic fungi in Taxus chinensis var. mairei].[南方红豆杉根的微观结构及内生真菌的分布]
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Jun;28(3):372-4.
8
ISOLATION AND IDENTIFICATION OF AN ENDOPHYTIC FUNGUS PRODUCING PACLITAXEL FROM TAXUS WALLICHIANA VAR MAIREI.从南方红豆杉中分离和鉴定一株产紫杉醇的内生真菌
Nutr Hosp. 2015 Dec 1;32(6):2932-7. doi: 10.3305/nh.2015.32.6.9781.
9
Secondary metabolites of Xylaria sp., an endophytic fungus from Taxus mairei.来自南方红豆杉的一种内生真菌——炭角菌属的次生代谢产物。
Nat Prod Res. 2016 Nov;30(21):2442-7. doi: 10.1080/14786419.2016.1198350. Epub 2016 Jun 30.
10
Screening of taxol-producing endophytic fungi from Taxus chinensis var. mairei.从南方红豆杉中筛选产紫杉醇内生真菌。
Prikl Biokhim Mikrobiol. 2007 Jul-Aug;43(4):490-4.

引用本文的文献

1
Clinical application of targeted nanopore sequencing in pathogen detection in patients with sepsis.靶向纳米孔测序在脓毒症患者病原体检测中的临床应用
BMC Infect Dis. 2025 Feb 24;25(1):259. doi: 10.1186/s12879-025-10604-3.

本文引用的文献

1
Comparison of Bioactive Compounds and Antioxidant Activities in Differentially Pigmented Fruits.不同颜色果实中生物活性化合物和抗氧化活性的比较。
Molecules. 2023 Aug 27;28(17):6272. doi: 10.3390/molecules28176272.
2
Diversity of Endophytic Microbes in and Their Potential for Plant Growth Promotion and Taxane Accumulation.植物内生微生物的多样性及其促进植物生长和紫杉烷积累的潜力
Microorganisms. 2023 Jun 23;11(7):1645. doi: 10.3390/microorganisms11071645.
3
Alternaria alternata F3, a Novel Taxol-Producing Endophytic Fungus Isolated from the Fruits of Taxus cuspidata: Isolation, Characterization, Taxol Yield Improvement, and Antitumor Activity.
短梗霉 F3，一种新型的来源于南方红豆杉果实的紫杉醇产生内生真菌：分离、鉴定、紫杉醇产量提高和抗肿瘤活性。
Appl Biochem Biotechnol. 2024 Apr;196(4):2246-2269. doi: 10.1007/s12010-023-04661-0. Epub 2023 Jul 27.
4
Endophytic fungus sp. AP12 promotes growth physiology and andrographolide biosynthesis in (Burm. f.) Nees.内生真菌sp. AP12促进穿心莲（爵床科穿心莲属）的生长生理及穿心莲内酯生物合成。
Front Plant Sci. 2023 Jul 4;14:1166803. doi: 10.3389/fpls.2023.1166803. eCollection 2023.
5
Melatonin enhances biomass, phenolic accumulation, and bioactivities of rosemary (Rosmarinus officinalis) in vitro shoots under UV-B stress.褪黑素可增强紫外线B胁迫下迷迭香（Rosmarinus officinalis）离体芽的生物量、酚类物质积累及生物活性。
Physiol Plant. 2023 Jul-Aug;175(4):e13956. doi: 10.1111/ppl.13956.
6
The influences of fungal endophytes inoculation on the biochemical status of grape cells of different varieties in vitro.真菌内生菌接种对不同品种葡萄细胞体外生化状态的影响。
Plant Biotechnol (Tokyo). 2022 Dec 25;39(4):335-343. doi: 10.5511/plantbiotechnology.22.0730a. Epub 2022 Nov 30.
7
Mass spectrometry imaging and single-cell transcriptional profiling reveal the tissue-specific regulation of bioactive ingredient biosynthesis in Taxus leaves.质谱成像和单细胞转录组分析揭示了紫杉叶片中生物活性成分生物合成的组织特异性调控。
Plant Commun. 2023 Sep 11;4(5):100630. doi: 10.1016/j.xplc.2023.100630. Epub 2023 May 25.
8
Integrated mass spectrometry imaging and single-cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems.整体质谱成像和单细胞转录组图谱策略为研究南方红豆杉茎中紫杉烷生物合成和运输提供了新的见解。
Plant J. 2023 Sep;115(5):1243-1260. doi: 10.1111/tpj.16315. Epub 2023 Jun 2.
9
Postharvest UV-B exposure drives changes in primary metabolism, phenolic concentration, and volatilome profile in berries of different grape (Vitis vinifera L.) varieties.采后 UV-B 辐射会引起不同葡萄（Vitis vinifera L.）品种浆果中初级代谢物、酚类浓度和挥发物谱的变化。
J Sci Food Agric. 2023 Oct;103(13):6340-6351. doi: 10.1002/jsfa.12708. Epub 2023 May 31.
10
Drought stress-mediated differences in phyllosphere microbiome and associated pathogen resistance between male and female poplars.干旱胁迫介导的雌雄杨树叶片微生物组差异及其与病原菌抗性的关系。
Plant J. 2023 Aug;115(4):1100-1113. doi: 10.1111/tpj.16283. Epub 2023 Jul 5.