Melatonin enhances biomass, phenolic accumulation, and bioactivities of rosemary (Rosmarinus officinalis) in vitro shoots under UV-B stress.

Melatonin is a stress-related hormone that plays a critical role in triggering the plant defence system and regulating secondary metabolism when plants are exposed to stress. To explore the potential roles of melatonin in response to Ultraviolet-B (UV-B) radiation, we examined the effects of exogenous melatonin on rosemary in vitro shoots under UV-B stress. The application of melatonin (50 μM) alleviated the adverse effects of UV-B stress on the biomass, photosynthetic pigment contents, and membrane lipids of the rosemary in vitro shoots. Melatonin significantly increased superoxide dismutase (1.15.1.1, SOD), peroxidase (1.11.1.7, POD), and catalase (1.11.1.6, CAT) activities by 62%, 99%, and 53%, respectively. The contents of total phenols, rosmarinic acid, and carnosic acid increased under UV-B stress, and they further increased by the melatonin treatment by 41%, 68%, and 67%, respectively, compared with the control group. Under UV-B stress, the increased total phenol content in melatonin-pretreated plants could be attributed to the activation of phenylalanine ammonia-lyase (4.3.1.5, PAL) and tyrosine aminotransferase (2.6.1.5, TAT). In addition, melatonin enhanced the antioxidant and antibacterial activities of the rosemary in vitro shoots under UV-B stress. These results suggest that melatonin can alleviate the damage caused by UV-B stress and also enhance the secondary metabolism and bioactivity of rosemary in vitro shoots.