College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
Kharkiv Institute, Hangzhou Normal University, Hangzhou, 311121, China.
BMC Microbiol. 2024 Aug 3;24(1):291. doi: 10.1186/s12866-024-03445-8.
Taxol, derived from Taxus trees, is a valuable natural resource for the development of anticancer drugs. Endophytic fungi from Taxus trees are a promising alternative source of Taxol. However, the impact of plant-endophytic microbial interaction on the host's Taxol biosynthesis is largely unknown.
In the current study, the diversity of endophytic fungi in three different Taxus species was analyzed using Internal Transcribed Spacer sequencing. A total of 271 Operational Taxonomic Units (OTUs) were identified, grouping into 2 phyla, 8 classes, 16 orders, 19 families, and 19 genera. Alpha and beta diversity analysis indicated significant differences in endophytic fungal communities among the various Taxus trees. At the genus level, Alternaria and Davidiella were predominantly found in T. mairei and T. media, respectively. By utilizing a previously published dataset, a Pearson correlation analysis was conducted to predict the taxol biosynthesis-related fungal genera. Following screening, two isolates of Alternaria (L7 and M14) were obtained. Effect of inoculation with Alternaria isolates on the gene expression and metabolite accumulation of T. mairei was determined by transcriptomic and untargeted metabolomic studies. The co-inoculation assay suggests that the two Alternaria isolates may have a negative regulatory effect on taxol biosynthesis by influencing hormone signaling pathways.
Our findings will serve as a foundation for advancing the production and utilization of Taxus and will also aid in screening endophytic fungi related to taxol production.
紫杉醇来源于紫杉属植物，是开发抗癌药物的宝贵自然资源。紫杉属植物的内生真菌是紫杉醇的有前途的替代来源。然而，植物-内生微生物相互作用对宿主紫杉醇生物合成的影响在很大程度上是未知的。
在本研究中，使用内部转录间隔区测序分析了三种不同紫杉属植物的内生真菌多样性。共鉴定出 271 个操作分类单元（OTUs），分为 2 个门、8 个纲、16 个目、19 个科和 19 个属。α和β多样性分析表明，不同紫杉属植物的内生真菌群落存在显著差异。在属水平上，交替单胞菌属和戴维氏菌属分别主要存在于南方红豆杉和密叶红豆杉中。利用先前发表的数据集，进行 Pearson 相关性分析以预测与紫杉醇生物合成相关的真菌属。筛选后，获得了两种交替单胞菌属（L7 和 M14）的分离株。通过转录组学和非靶向代谢组学研究，确定了接种交替单胞菌属分离株对南方红豆杉基因表达和代谢物积累的影响。共接种试验表明，两种交替单胞菌属分离株可能通过影响激素信号通路对紫杉醇生物合成产生负调节作用。
我们的研究结果将为推进紫杉属植物的生产和利用提供基础，并有助于筛选与紫杉醇生产相关的内生真菌。
