State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
College of Animal Science and Veterinary Medicine, Henan institute of Science and Technology, Xinxiang 453003,China.
Poult Sci. 2024 Nov;103(11):104251. doi: 10.1016/j.psj.2024.104251. Epub 2024 Aug 22.
This study aimed to investigate the effects of baicalin and chlorogenic acid (BC) on growth performance, intestinal barrier function, antioxidant capacity, intestinal microbiota, and mucosal metabolism in broilers. A total of 720 twenty-one-day-old broilers were randomly allocated into 3 groups, with 6 replicates per group and 40 chickens per replicate. They were fed a basal diet (Con group) or a basal diet supplemented with 250 or 400 mg/kg BC (BC250 and BC400 groups) for 40 consecutive days. The results revealed that 250 mg/kg BC significantly increased 60-d body weight and average daily gain during 39 to 60 d (P < 0.05). Furthermore, Supplementation with 250 mg/kg BC improved the antioxidant capacity and immunity of broilers, as evidenced by increased (P < 0.05) superoxide dismutase and decreased (P < 0.05) malondialdehyde levels in serum and ileum, as well as increased (P < 0.05) immunoglobulin G levels. Supplementation with 250 mg/kg BC enhanced intestinal development by improving intestinal morphology and promoting the proliferation of intestinal crypts. Moreover, Supplementation with 250 mg/kg BC improved (P < 0.05) intestinal permeability, up-regulated (P < 0.05) the expression of tight junction-related genes (Occludin and ZO-1), and down-regulated (P < 0.05) the expression of pro-inflammatory genes (IL-2, IL-8, and IFN-γ). 16S rRNA sequencing revealed significant enrichment of Microbacteriaceae, Micromonosporaceae, Anaerovoracaceae, and Coriobacteriaceae in the BC250 group. Metabolomics showed that 250 mg/kg BC up-regulated the lysosome, foxo signaling pathway, glycosylphosphatidylinositol (GPI)-anchor biosynthesis, and oxidative phosphorylation pathways, while down-regulating the biosynthesis of cofactors pathway. In conclusion, supplementing diets with 250 mg/kg BC is recommended to modulate intestinal microbiota, mucosal metabolism, and antioxidant capacity, thereby improving broiler growth performance and intestinal health.
本研究旨在探讨黄芩苷和绿原酸（BC）对肉鸡生长性能、肠道屏障功能、抗氧化能力、肠道微生物群和黏膜代谢的影响。将 720 只 21 日龄的肉鸡随机分为 3 组，每组 6 个重复，每个重复 40 只鸡。它们分别饲喂基础日粮（Con 组）或基础日粮添加 250 或 400 mg/kg BC（BC250 和 BC400 组），连续 40 天。结果表明，250 mg/kg BC 显著提高了 60 日龄体重和 39 至 60 日龄的平均日增重（P<0.05）。此外，250 mg/kg BC 提高了肉鸡的抗氧化能力和免疫力，血清和回肠中超氧化物歧化酶水平升高（P<0.05），丙二醛水平降低（P<0.05），免疫球蛋白 G 水平升高（P<0.05）。250 mg/kg BC 通过改善肠道形态和促进肠隐窝增殖来增强肠道发育。此外，250 mg/kg BC 改善了肠道通透性（P<0.05），上调了紧密连接相关基因（Occludin 和 ZO-1）的表达（P<0.05），下调了促炎基因（IL-2、IL-8 和 IFN-γ）的表达（P<0.05）。16S rRNA 测序显示，BC250 组中 Microbacteriaceae、Micromonosporaceae、Anaerovoracaceae 和 Coriobacteriaceae 显著富集。代谢组学表明，250 mg/kg BC 上调了溶酶体、Foxo 信号通路、糖基磷脂酰肌醇（GPI）锚生物合成和氧化磷酸化通路，下调了辅因子生物合成通路。综上所述，建议在日粮中添加 250 mg/kg BC 来调节肠道微生物群、黏膜代谢和抗氧化能力，从而改善肉鸡的生长性能和肠道健康。
