Kivrak Ezgi, Kara Pinar
Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, 35100 Izmir, Bornova, Turkey; Graduate School of Natural and Applied Sciences, Department of Biomedical Technologies, Ege University, 35100 Izmir, Bornova, Turkey.
Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, 35100 Izmir, Bornova, Turkey.
Bioelectrochemistry. 2025 Feb;161:108806. doi: 10.1016/j.bioelechem.2024.108806. Epub 2024 Sep 5.
Ovarian cancer, known as "silent killer", is a gynocological cancer with high mortality that usually diagnosed in the late stages. Gold standard immunoassay technique is evaluation of CA-125 levels which is not merely specific to ovarian cancer. Therefore, there is a need for sensitive determination of more specific biomarkers. miR-200 family is RNA nucleic acids that known to be upregulated in the presence of ovarian cancer. Since diagnosis based on a single biomarker is prone to generate misleading results, it is important to develop point-of-care systems that allow diagnosis of multiple miRNAs. Herein, an electrochemical nanobiosensor platform was developed for the multiplexed and simultaneous detection of miR-200c and miR-141. Biorecognition part was constitutued of methylene blue and ferrocene labeled hairpin DNA probes immobilized onto carboxylated graphene oxide modified pencil graphite electrodes. Their hybridization with miRNAs were examined upon "signal-off" approach using Square Wave Voltammetry. The platform demonstrated a linear detection range of 0.1 pM to 10 nM for both miR-141 and miR-200c, with low detection limits of 0.029 pM and 0.026 pM, respectively. We assume that the developed biosensor platform may pave the way in early diagnosis of the disease and the development of more effective treatment strategies.
卵巢癌被称为“沉默杀手”，是一种死亡率很高的妇科癌症，通常在晚期才被诊断出来。金标准免疫测定技术是评估CA - 125水平，但CA - 125并非仅对卵巢癌具有特异性。因此，需要灵敏地测定更具特异性的生物标志物。miR - 200家族是已知在卵巢癌存在时上调的RNA核酸。由于基于单一生物标志物的诊断容易产生误导性结果，开发能够诊断多种miRNA的即时检测系统很重要。在此，开发了一种电化学纳米生物传感器平台，用于多重同时检测miR - 200c和miR - 141。生物识别部分由固定在羧基化氧化石墨烯修饰铅笔石墨电极上的亚甲基蓝和二茂铁标记的发夹DNA探针组成。使用方波伏安法在“信号关闭”方法下检测它们与miRNA的杂交情况。该平台对miR - 141和miR - 200c的线性检测范围均为0.1 pM至10 nM，检测限分别低至0.029 pM和0.026 pM。我们认为，所开发的生物传感器平台可能为该疾病的早期诊断以及更有效治疗策略的开发铺平道路。
