Food Ingredients and Biopolymers Laboratory, Department of Plant Sciences, North Dakota State University, Fargo, ND 58102, United States; Department of Food Science and Technology, Food Innovation Center, University of Nebraska, Lincoln, NE 68508, United States.
Food Ingredients and Biopolymers Laboratory, Department of Plant Sciences, North Dakota State University, Fargo, ND 58102, United States.
Int J Biol Macromol. 2024 Nov;279(Pt 3):135395. doi: 10.1016/j.ijbiomac.2024.135395. Epub 2024 Sep 6.
This study aims to fabricate high internal phase emulsion gels (HIPEGs) using modified hemp protein isolates for microencapsulating cannabidiol (CBD) to enhance their chemical stability and bioaccessibility. Importantly, the combined effect of CBD concentrations (0.1 vs 0.5 wt%) and post gel storage conditions (before-refrigeration (BR) vs after-refrigeration (AR)) on the properties of HIPEGs were investigated. The results showed that the CBD concentration above 0.4 % is necessary to fabricate a stable HIPEG. The rheological properties of HIPEGs were influenced by CBD concentration and refrigeration. The AR gels with 0.5 % CBD showed the highest gel strength (up to 91.7 Pa) and solid-like structures. These properties allowed to HIPEGs maintain good physical stability during storage at 4, 25, and 37 °C for 14 days due to the interconnected polyhedral protein matrices and thick interfacial protein layers. These unique protein architectures offered superior protection against CBD degradation (<2 % of initial added amount) for 100 days during exposure to light and temperature (25 or 37 °C). The INFOGEST digestion results showed the BR gels effectively protected CBD during digestion and consequently improved their stability and bioaccessibility up to 95 % and 74 %, respectively. Overall, the fabricated HIPEGs could be valuable for nutraceutical delivery.
本研究旨在使用改良的麻蛋白分离物制备高内相比乳液凝胶（HIPEGs），以包埋大麻二酚（CBD），从而提高其化学稳定性和生物利用度。重要的是，研究了 CBD 浓度（0.1 与 0.5wt%）和凝胶后储存条件（冷藏前（BR）与冷藏后（AR））对 HIPEGs 性质的综合影响。结果表明，需 CBD 浓度高于 0.4%才能制备稳定的 HIPEG。HIPEGs 的流变性能受 CBD 浓度和冷藏的影响。含 0.5% CBD 的 AR 凝胶具有最高的凝胶强度（高达 91.7Pa）和固态结构。这些特性使 HIPEG 在 4、25 和 37°C 下储存 14 天时能够保持良好的物理稳定性，这是由于多面体形蛋白质基质和厚的界面蛋白质层的相互连接。这些独特的蛋白质结构为 CBD 降解提供了卓越的保护（<初始添加量的 2%），在暴露于光和温度（25 或 37°C）的 100 天内。INFOGEST 消化结果表明，BR 凝胶在消化过程中有效地保护了 CBD，从而将其稳定性和生物利用度分别提高至 95%和 74%。总的来说，所制备的 HIPEGs 可能对营养保健品的输送具有重要价值。
