Biobased Chemistry and Technology, Wageningen University and Research, Bornse Weilanden 9, PO Box 17, 6708 WG Wageningen, the Netherlands; Food Process Engineering, Wageningen University and Research, Bornse Weilanden 9, PO Box 17, 6708 WG Wageningen, the Netherlands.
Biobased Chemistry and Technology, Wageningen University and Research, Bornse Weilanden 9, PO Box 17, 6708 WG Wageningen, the Netherlands.
Food Res Int. 2024 Nov;195:114948. doi: 10.1016/j.foodres.2024.114948. Epub 2024 Aug 22.
Oleosomes are natural lipid droplets that can be extracted intact from oil seeds, forming oil/water emulsions. Their lipid cores, surrounded by a monolayer of phospholipids and proteins, make oleosomes suitable as carriers of hydrophobic bioactive compounds like cannabidiol (CBD). As CBD is crystalline at room temperature, it first has to be liquified to allow better encapsulation. This was done by heating (80 °C for 4 h) or by pre-solubilizing CBD in ethanol and then the liquified CBD was mixed with oleosome dispersions for the encapsulation. Both methods exhibit good encapsulation efficiency, but the results were significantly influenced by the ratio of CBD to lipid contents, regardless of the encapsulation method applied. At higher concentrations of CBD relative to that of the lipid in the oleosomes, the encapsulation efficiency decreased as saturation was attained. Moreover, the in vitro digestion analysis was conducted to investigate the potential of oleosomes as carriers to transport CBD. The relatively slow and steady release of CBD from oleosomes indicates that oleosomes are a slow-release carrier for hydrophobic functional ingredients. An important finding is that the encapsulation and in vitro digestive properties of the oleosomes remain unaffected by the presence of CBD, heating treatment or ethanol, which could bring more opportunities for the applications of oleosomes as carriers in various fields.
油体是可从油籽中完整提取形成油/水乳液的天然脂质体。它们的脂质核心被一层磷脂和蛋白质包围，使油体成为大麻二酚 (CBD) 等疏水性生物活性化合物的理想载体。由于 CBD 在室温下为结晶状，首先必须将其液化以实现更好的包封。通过加热（80°C 持续 4 小时）或预先将 CBD 溶解在乙醇中，然后将液化的 CBD 与油体分散体混合进行包封，从而实现 CBD 的液化。这两种方法都表现出良好的包封效率，但结果都受到 CBD 与脂质含量比例的显著影响，而与应用的包封方法无关。当 CBD 的浓度相对于油体中的脂质浓度较高时，由于达到饱和，包封效率降低。此外，还进行了体外消化分析，以研究油体作为载体输送 CBD 的潜力。CBD 从油体中缓慢而稳定的释放表明，油体是疏水性功能性成分的缓释载体。一个重要的发现是，CBD 的存在、热处理或乙醇对油体的包封和体外消化特性没有影响，这为油体作为载体在各个领域的应用带来了更多机会。
