Madsen P S
Nord Vet Med. 1979 Nov;31(11):449-54.
Milk somatic cell counting was carried out by Fossomatic and microscopically. In unpreserved milk samples Fossomatic counts increased by up to 100% during the first 24 hours after milking. During the next 24 hours the counts increased by 5% whereafter they remained stable until at least 80 hours after milking, when the samples were stored at 5 degress C. On microscopical counting using methylene blue for staining, the results were stable from shortly after milking. In counting, attention had to be paid to the fact that within the first 24 hours a certain part of the cells would stain but faintly. After preservation with potassium bichromate the Fossomatic counts increased more rapidly. Stable results were found 5--8 hours after preservation. The final level of the Fossomatic counts was found to be app. 10% higher in preserved than in unpreserved samples. A smaller increase was found by microscopic counting. The rise of the cell counts during the first 24 hours after milking is probably due to inadequate stainability of living cells with ethidium bromide, resulting in a certain part of them being recorded by the Fossomatic. During the first day the vitality of the cells diminishes whereby they become stainable and countable. This process in hastened by potassium bichromate treatment.
牛奶体细胞计数通过Fossomatic仪器和显微镜进行。在未保存的牛奶样本中，挤奶后的头24小时内，Fossomatic计数增加了高达100%。在接下来的24小时内，计数增加了5%，此后保持稳定，直到挤奶后至少80小时，此时样本储存在5摄氏度。使用亚甲蓝染色进行显微镜计数时，挤奶后不久结果就稳定了。计数时必须注意，在头24小时内，一定比例的细胞染色较淡。用重铬酸钾保存后，Fossomatic计数增加得更快。保存5至8小时后得到稳定结果。发现保存样本的Fossomatic计数最终水平比未保存样本约高10%。显微镜计数发现增加幅度较小。挤奶后头24小时内细胞计数的增加可能是由于活细胞对溴化乙锭的染色性不足，导致其中一部分被Fossomatic记录。在第一天，细胞活力下降，从而变得可染色和可计数。重铬酸钾处理加速了这个过程。
