Gonzalo C, Baro J A, Carriedo J A, Primitivo F S
Departamento de Producción Animal, Universidad de León, Spain.
J Dairy Sci. 1993 Jan;76(1):115-9. doi: 10.3168/jds.S0022-0302(93)77330-0.
The Fossomatic method for SCC was compared with the direct microscopic method in 85 half-udder samples of sheep milk. The correlation coefficient was .986. The repeatability of the Fossomatic method showed average variation coefficients less than 5%. The carry-over effect between samples was less than 5%. The effect of the storage method (fresh milk, refrigerated at 4 degrees C and frozen at -19 degrees C) and the sample age were studied in 48 samples of foremilk and strippings. The storage method had a significant effect on the SCC variation. The average fresh, refrigerated, and frozen sample counts were 125,000, 110,000, and 82,000 cells/ml for foremilk and 201,000, 192,000, and 145,000 cells/ml for strippings, respectively. The effect of age on the refrigerated samples was also significant; counts were reduced by about 14% from d 1 to 7 in both types of milk. The effect of age on the frozen sample varied. These results suggest standardization of age and storage conditions of the milk samples to reduce variation of SCC. The milk must not be frozen.
在85份羊奶半乳房样本中，将用于体细胞计数（SCC）的Fossomatic方法与直接显微镜检查法进行了比较。相关系数为0.986。Fossomatic方法的重复性显示平均变异系数小于5%。样本间的残留效应小于5%。在48份初乳和末乳样本中研究了储存方法（新鲜牛奶、4℃冷藏和-19℃冷冻）和样本保存时长的影响。储存方法对体细胞计数的变化有显著影响。初乳的新鲜、冷藏和冷冻样本的平均细胞数分别为125,000、110,000和82,000个/毫升，末乳分别为201,000、192,000和145,000个/毫升。保存时长对冷藏样本的影响也很显著；两种类型的牛奶从第1天到第7天细胞数均减少了约14%。保存时长对冷冻样本的影响各不相同。这些结果表明，应对牛奶样本的保存时长和储存条件进行标准化，以减少体细胞计数的变化。牛奶不得冷冻。
